Veterinary Research Forum
Vet Res Forum

Effect of curcumin-loaded polycaprolactone scaffold on Achilles tendon repair in rats

Document Type : Original Article

Authors

Narges Sufian 1
Mehdi Behfar 2
Rahim Hobbenaghi 3
Siamak Asri-Rezaei 4

1 DVSc Candidate, Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

4 Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2024.2029563.4286
Abstract
Scaffolds play a crucial role in tendon healing by providing structural support, promoting cell infiltration, and guiding tissue regeneration. Polycaprolactone (PCL) has been used as a polymer in biological scaffolds for several tissue engineering studies. This study aimed to investigate the effects of curcumin-loaded PCL scaffold on Achilles tendon using a tenotomy model in rats. Twenty adult male Wistar rats were randomized into two groups. In control group, tenotomy and suture placement were performed. The identical intervention followed by the implantation of curcumin-loaded PCL scaffold around the tendon stumps was performed in the treatment group. The nanofibrous PCL scaffold containing 5.00% curcumin was fabricated by electrospinning. Walking track analysis was performed weekly. Then, after 6 weeks, histopathological examination and tendon mechanical tests were performed. The weekly walking track analysis revealed a significant improvement in Achilles functional index in scaffold-treated rats from week three to six. The rate of functional improvement was remarkably slower in the control group. Histopathological examination revealed aseptic inflammation and enhanced neovascularization in the treatment group. Also, collagen arrangement and density were significantly improved in this group compared to the control samples including less regular orientation and loose organization of collagen fibers. Significant increase in mechanical properties, except for strain, was observed in the treatment group. The present study demonstrated that implantation of curcumin-loaded PCL scaffold resulted in increased fibrillar architecture, as well as improved mechanical properties and Achilles functional index in rats. To reduce the biodegradation-induced inflammation, an anti-inflammatory treatment is recommended.

Keywords

Collagen
Curcumin
Scaffold
Tendon
Tensile strength

Subjects

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Veterinary Research Forum
Volume 15, Issue 11
November 2024
Pages 621-627

  • Receive Date 18 May 2024
  • Revise Date 22 June 2024
  • Accept Date 29 June 2024

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