Veterinary Research Forum
Vet Res Forum

Viability of guinea pig (Cavia porcellus) spermatozoa diluted in Tris-buffer extenders stored at 5.00 ˚C

Document Type : Original Article

Authors

Mozhdeh Heydari 1
Alaleh Rakhshanpour 2
Ramin Mazaheri Khameneh 3
Ali Soleimanzadeh 4

1 DVM Graduate, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Clinical Pathology and Internal Medicine, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

4 Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2024.2023686.4186
Abstract
The cooling procedure markedly diminishes the quality of guinea pig (Cavia porcellus) sperms, primarily because their membranes are highly susceptible to this process. This susceptibility triggers the generation of reactive oxygen species and free radicals, ultimately leading to lipid peroxidation in the sperm membrane. Surprisingly, there has been a lack of research on the use of Tris-based extenders to safeguard guinea pig sperm under refrigeration conditions. This study aimed to assess the viability of guinea pig spermatozoa diluted in Tris buffer-based extenders during storage at 5.00 ˚C. Sperm collection was carried out through castration of the animals. For this study, 10 adult male guinea pigs were utilized, being divided into four groups including phosphate-buffered saline (PBS), human tubal fluid (HTF), Tris-citric-glucose (TCG), and Tris-fructose-yolk (TFY) cultures. Evaluations including sperm motility, morphology, plasma membrane integrity, viability, and total count were conducted at 0, 24, and 48 hr after sampling. The results obtained indicated that at the 24-hr and 48-hr marks of the experiment, both overall and progressive motility percentages, viability, plasma membrane integrity, and morphology of sperms in the PBS and HTF cultures exhibited a significant increase in comparison with the TCG and TFY cultures. Consequently, it can be inferred that PBS and HTF cultures are highly effective in preserving the quality of guinea pig spermatozoa.

Keywords

Guinea pig
Semen preservation
Sperm parameters

Subjects

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Veterinary Research Forum
Volume 15, Issue 12
December 2024
Pages 691-696

  • Receive Date 24 February 2024
  • Revise Date 11 June 2024
  • Accept Date 29 June 2024

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