Veterinary Research Forum
Vet Res Forum

Proteomic profiling of pseudorabies virus-infected PK-15 cells based on 4D label free analysis

Document Type : Original Article

Authors

Shuaiwei Wang 1
Xiaoyong Chen 2
Houqiang Luo 1
Meng Wang 1
Junyu Liu 1
Qingsong Han 1

1 Department of Animal Science, Wenzhou Vocational College of Science and Technology, Wenzhou, China

2 Department of Science, College of Science, Zhejiang Normal University, Jinhua, China

https://doi.org/10.30466/vrf.2024.2026963.4239
Abstract
Pseudorabies virus (PRV) heavily depends on host machinery to support its life cycle. Investigating the interaction between PRV and host could aid in the understandings of viral pathogenesis. In this study, we performed a 4D label free proteomic method to examine the differentially expressed proteins in porcine kidney PK-15 cells with PRV infection. The results showed that the levels of 661 proteins were significantly elevated and 693 proteins were markedly reduced. Furthermore, these altered proteins were primarily enriched in spliceosome, protein processing in endoplasmic reticulum (ER), RNA transport, and protein export. To ensure the reliability of the proteomic results, the protein levels of formin binding protein 11 and wolfram syndrome 1as components of spliceosome and ER were verified via western blotting and the results were consistent. Together, our data shed light on a new protein profiling induced by PRV infection and highlighted the importance of spliceosome and ER in PRV replication which could promote understandings of host-PRV interplay.

Keywords

Endoplasmic reticulum
Herpes virus
Proteomic analysis
Spliceosome
Subjects
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Veterinary Research Forum
Volume 16, Issue 3
March 2025
Pages 141-147

  • Receive Date 28 April 2024
  • Accept Date 29 June 2024

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