Veterinary Research Forum

Antibiotic resistance dynamics of some common probiotic Lactobacillus species and avian pathogenic Escherichia coli in colibacillosis-diseased versus healthy broiler chickens

Document Type : Original Article

Authors

Nimra Khalid 1
Syed Mohsin Bukhari 1
Waqas Ali 1
Ali Ahmad Sheikh 2

1 Department of Wildlife and Ecology, Faculty of Fisheries and Wildlife, University of Veterinary and Animal Sciences, Lahore, Pakistan

2 Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan

https://doi.org/10.30466/vrf.2025.2037056.4381
Abstract
This study focuses on understanding how the sensitivity patterns of common probiotic Lactobacillus species differ between healthy and colibacillosis-diseased broiler chickens and explores any correlation between these patterns and avian pathogenic Escherichia coli (APEC). Ileum samples were collected from the healthy (n = 10) and colibacillosis-diseased (n = 10) broiler poultry farms. Lactobacillus and E. coli were identified and incidence rate of six probiotic Lactobacillus species (L. acidophilus, L. brevis, L. casei, L. plantrum, L. delbrueckii, and L. fermenti) was determined, and their susceptibility to different antibiotics (ampicillin, chloramphenicol, erythromycin, kanamycin, tetracycline, and vancomycin) was tested. A total of 120 Lactobacilli were examined. Certain Lactobacillus spp. from healthy birds (L. brevis) and those affected by colibacillosis (L. acidophilus) showed better sensitivity and lower multiple antibiotic resistance to chloramphenicol, erythromycin, and kanamycin. Statistical analysis revealed significant correlations between ampicillin and chloramphenicol, ampicillin and erythromycin, ampicillin and tetracycline, and ampicillin and vancomycin resistance. Also, a significant correlation was observed between erythromycin and tetracycline, and kanamycin and vancomycin. All APEC strains showed multiple antibiotic resistance. A strong positive correlation was found between resistant Lactobacillus spp. and APEC isolates from diseased birds, indicating co-occurrence of antibiotic resistance. The study suggests that antibiotic resistance in APEC and Lactobacillus species may spread through horizontal transfer, highlighting the need for improved anti-microbial management in poultry systems.

Keywords

Chickens
Drug Resistance
Escherichia coli Infections
Probiotics
Subjects
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Veterinary Research Forum
Volume 16, Issue 7
July 2025
Pages 391-397

  • Receive Date 29 July 2024
  • Revise Date 01 January 2025
  • Accept Date 01 February 2025

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