Veterinary Research Forum
Vet Res Forum

Rotavirus infection in cattle

Document Type : Short Communication

Authors

Yahia Hassan Ali 1
Intisar Kamil Saeed 1
Muaz Magzoub Abdellatif 1
Amani Ahmed Ali 2
Alaa Ahmed Mustafa 3
Hind Abdel-Mageed Rikabi 4
Husham Mohammed Al-Hasssan Ataalfadeel 3

1 Department of Biological Sciences, College of Science, Northern Border University, Arar, Saudi Arabia

2 Department of Pharmacology and Toxicology, College of Pharmacy, Northern Border University, Arar, Saudi Arabia

3 Department of Mathematics, College of Science, Northern Border University, Arar, Saudi Arabia

4 Department of Pharmacy Practice, College of Pharmacy, Northern Border University, Arar, Saudi Arabia

https://doi.org/10.30466/vrf.2025.2044037.4506
Abstract
Rotavirus is one of the major causes of diarrhea in different animal species and has a bad economic impact due to the losses in neonates and productivity. To investigate the occurrence of this infection in bovine calves, three localities in Khartoum State, Sudan, were selected. A total of 200 fecal samples were collected from diarrheic calves; 100 from Khartoum and 50 from each of Khartoum Bahari and Omdurman provinces. Collected samples were screened for a group A rotavirus antigen using enzyme-linked immunosorbent assay (ELISA). Positive results were seen in 40.00% of samples; the highest prevalence of 42.00% was found in samples from Khartoum province. Five ELISA-positive samples were examined under electron microscope, and characteristic wheel-like appearance of rotavirus was visualized. Polyacrylamide gel electrophoresis was also applied on 15 of the positive samples; eight samples showed different polyacrylamide gel electrophoretic group A rotavirus long profile with different patterns. The results showed that the occurrence of rotavirus infection in cattle in Khartoum State is increasing.

Keywords

Cattle
Electron microscopy
ELISA
Group A rotavirus
Polyacrylamide gel electrophoresis

Subjects

1.     Geletu US, Usmael MA, Bari FD. Rotavirus in calves and its zoonotic importance. Vet Med Int 2021; 2021: 6639701. doi: 10.1155/2021/6639701.
2.     Matthijnssens J, Desselberger U. Genome diversity and evolution of rotaviruses. In: Hacker J, Dobrindt U, Kurth R (Eds). Genome plasticity and infectious diseases.1st ed. Washington DC, USA: ASM Press 2012: 214-241.
3.     Chen S, Zhang W, Zhai J, et al. Prevalence of bovine rotavirus among cattle in mainland China: a meta-analysis. Microb Pathog 2022: 170: 105727. doi: 10.1016/j.micpath.2022.105727.
4.     Makwana PM, Kalyani IH, Desai D, et al. Detection of bovine rotavirus (BRV) infection in neonatal calves of in and around Navsari district of South Gujarat, India. J Entomol Zool Stud 2020; 8(2): 1092-1097.
5.     Alotaibi MA, Al-Amad S, Chenari Bouket A, et al. High occurrence among calves and close phylogenetic relationships with human viruses warrants close surveillance of rotaviruses in Kuwaiti dairy farms. Front Vet Sci 2022; 9: 745934. doi: 10.3389/ fvets.2022.745934.
6.     Pourasgari F, Kaplon J, Karimi-Naghlani S, et al. The molecular epidemiology of bovine rotaviruses circulating in Iran: a two-year study. Arch Virol 2016; 161(12): 3483-3494.
7.     Yilmaz V. Investigation of rotavirus infection in calves with diarrhea in northeast Turkey. Anim Vet Sci 2016; 4(1): 1-4.
8.     Uhde FL, Kaufmann T, Sager H, et al. Prevalence of four enteropathogens in the faeces of young diarrhoeic dairy calves in Switzerland. Vet Rec 2008; 163(12): 362-366.
9.     Pisanelli G, Martella V, Pagnini U, et al. Distribution of G (VP7) and P (VP4) genotypes in buffalo group A rotaviruses isolated in Southern Italy. Vet Microbiol 2005; 110(1-2): 1-6.
10. Ammar SS, Mokhtaria K, Tahar BB, et al. Prevalence of rotavirus (GARV) and coronavirus (BCoV) associated with neonatal diarrhea in calves in western Algeria. Asian Pac J Trop Biomed 2014; 4(Suppl 1): S318-S322.
11. Abdel-Rady A, Zaitoun AM, Youssef ZM. Clinical study with rapid serological detection of rotavirus infection in diarrheic neonatal calves. Acta Sci Vet Sci 2022; 4(2): 29-33.
12. Seid U, Dawo F, Tesfaye A, et al. Isolation and characterization of coronavirus and rotavirus associated with calves in central part of Oromia, Ethiopia. Vet Med Int 2020; 2020: 8869970. doi: 10.1155/2020/8869970.
13. El Nour TM. Studies on neonatal diarrhea and pre-weaning calf mortality in a Friesian dairy herd. MSc Thesis. University of Khartoum. Khartoum, Sudan: 1994.
14. Ali YH, Khalafalla AI, Intisar KS, et al. Rotavirus infection in human and domestic animals in Sudan. J Sci Technol 2011; 12(4): 58-63.
15. Steele AD, Alexander JJ. Molecular epidemiology of rotavirus in black infants in South Africa. J Clin Microbiol 1987; 25(12): 2384-2387.
16. Aldawmy FK, Thwiny HT, Abo Almaali HM. Epidemiological and molecular study of rotavirus infection among human and animal in Karbala and Basrah provinces. Iraqi J Vet Sci 2021; 35(2): 403-140.
17. Suresh T, Rai RB, Wani MY, et al. Detection of bovine rotavirus in neonatal calf diarrhoea by ELISA, FAT and transmission electron microscopy. Int J Curr Res 2013; 5(7): 1935-1939.
18. Basera SS, Singh R, Vaid N, et al. Detection of rotavirus infection in bovine calves by RNA-PAGE and RT-PCR. Indian J Virol 2011; 21(2): 144-147.
19. Ali YH, Khalafalla AI, Gaffar ME, et al. Rotavirus-associated camel calf diarrhoea in Sudan. J Anim Vet Adv 2005; 4(3): 401-406.
Veterinary Research Forum
Volume 17, Issue 1
January 2026
Pages 63-65

  • Receive Date 28 October 2024
  • Revise Date 22 April 2025
  • Accept Date 23 April 2025

Home

Guide for Authors

Submit Manuscript

Reviewers

Contact Us