Veterinary Research Forum

Tannic acid ameliorates sperm quality and testicular damage in a rat model of ischemia-reperfusion

Document Type : Original Article

Authors

Hosna Ghaemi 1
Ali Soleimanzadeh 1
Rahim Mohammadi 2
Ali Shalizar-Jalali 3

1 Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2025.2072773.4928
Abstract
This study evaluated the protective effects of intraperitoneal (IP) tannic acid (TA) against ischemia-reperfusion (IR) injury in a rat model of testicular torsion. Eighteen mature male Wistar rats were randomized into three groups, including sham (surgery without ischemia), IR (3 hr ischemia + 3 hr reperfusion), and IR + TA (50.00 mg kg-1 TA; 100 µL intraperitoneally 30 min before reperfusion). Testicular tissues were sampled immediately after reperfusion for biochemical assays to measure malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels. Epididymides were analyzed 60 days later for sperm count, motility, viability, morphology, and DNA integrity. The IR significantly elevated MDA and sperm DNA damage while reducing SOD, GPx, sperm parameters, testicular weight, and spermatogenesis. The TA administration reversed these changes and restored the parameters to levels close to those of the sham group. Overall, intraperitoneal TA mitigated IR-induced oxidative stress and preserved reproductive function, indicating its potential therapeutic value in testicular torsion.

Keywords

Ischemia-reperfusion
Rat
Tannic acid
Testis
Subjects
Veterinary Research Forum
Volume 17, Issue 9
September 2026
Pages 639-645

  • Receive Date 26 September 2025
  • Revise Date 03 November 2025
  • Accept Date 15 November 2025

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