Veterinary Research Forum

First molecular and phylogenetic characterization of Physaloptera clausa in long-eared hedgehogs (Hemiechinus auritus) from Iran

Document Type : Short Communication

Authors

Aida Vafae Eslahi 1
Majid Pirestani 2
Moslem Karami 3
Sasan Khazaei 2
Milad Badri 1

1 Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

2 Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

3 Department of Biology, Faculty of science, University of Zanjan, Zanjan, Iran

https://doi.org/10.30466/vrf.2025.2057537.4719
Abstract
Physalopteridae nematodes pose a threat to a broad spectrum of animals including amphibians, reptiles, birds and mammals. The current study was the first molecular and phylogenetic characterization of Physaloptera clausa parasitizing long-eared hedgehogs (Hemiechinus auritus) in Iran. A male road-killed H. auritus was collected from Shahriar City, Tehran province in May 2022 and it was subjected to necropsy. After isolating parasites, they underwent morphological analysis using a light microscope and an identification key. For molecular analysis, the genomic DNA was isolated using the FavorPrepTM Tissue Genomic DNA Extraction Mini Kit. The PCR products were sequenced, the sequence data were analyzed and multiple alignments were conducted using the Clustal Omega. For phylogenetic analysis, these nucleotide sequences were aligned in MEGA 11 based on the lowest bayesian information criterion score. A cluster of parasites was found on the mucosa of the hedgehog stomach. All the nematodes were identified as P. clausa. The sequence obtained in this study has been submitted to GenBank® with the accession number OR088573. The phylogeny analysis revealed that the genus Physaloptera formed a major clade where P. clausa was clustered with various Physaloptera species, closely related to Turgida, Physalopteroides and Skrjabinoptera genera. Our study specified the necessity for additional taxonomic and phylogenetic research on Physaloptera species and related genera to fully understand the evolutionary dynamics and ecological significance of these parasites.

Keywords

Hemiechinus auritus
Molecular survey
Phylogenetic analysis
Physaloptera clausa
Subjects
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Veterinary Research Forum
Volume 17, Issue 4
April 2026
Pages 287-292

  • Receive Date 20 April 2025
  • Revise Date 29 May 2025
  • Accept Date 07 June 2025

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