Document Type : Original Article
Authors
1
Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar-125004, Haryana, India.
2
Lala Lajpat Rai University of Veterinary and Animal Sciences
3
Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar-125004, Haryana, India.
4
Livestock Farm Complex, Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar-125004, Haryana, India.
5
Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar-125004, Haryana, India
10.30466/vrf.2026.2059235.4742
Abstract
Porcine parvoviruses (PPVs) are globally recognized as significant contributors to porcine reproductive failure, primarily characterized by fetal death. It can cause infection in pregnant sows which can result in severe reproductive issues, including stillbirth, mummification, embryonic death, and infertility (SMEDI). An RPA assay targeting the variable region of the outer capsid protein gene of the PPV-7 genome was developed and systematically optimized under different reaction conditions. The assay exhibited optimal amplification performance at a constant temperature of 35 °C for 25 minutes, using 0.72 µM of each forward and reverse primer and 14 mM magnesium acetate (MgOAc). This assay demonstrated high sensitivity, detecting as few as 2050 copies of viral nucleic acids in both the conventional and fluorescent dye-based formats. The assay demonstrated high specificity, showing no cross-reactivity with other common porcine pathogens such as porcine sapelovirus, porcine circovirus, and classical swine fever virus.Of the 167 field samples tested, 23 were found to be positive for PPV-7, yielding a positivity rate of 13.7%. Operating at a low, constant temperature, the assay eliminates the requirement for advanced laboratory equipment, making it ideally suited for pen-side diagnostics in field conditions. In conclusion, this novel assay demonstrates strong potential for field-based detection of PPV-7 circulating within the swine population of Haryana, India, marking the first report of its kind from this region. Further validation using samples from clinically affected herds will strengthen its diagnostic applicability.
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