Veterinary Research Forum

Gastrodin mitigates testicular ischemia-reperfusion injury in rats

Document Type : Original Article

Authors

Si-Ming Wei 1, 2
Yu-Min Huang 3

1 Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China

2 School of Nursing, Zhejiang Chinese Medical University, Hangzhou, China

3 Department of Sports Science, College of Education, Zhejiang University, Hangzhou, China

https://doi.org/10.30466/vrf.2025.2064328.4805
Abstract
Testicular ischemia-reperfusion is accompanied by elevated production of reactive oxygen species. It has been reported that reactive oxygen species are highly reactive to cellular carbohydrates, DNA, lipids, and proteins, and result in testicular ischemia-reperfusion injury. Gastrodin is the principal active ingredient isolated from the medicinal plant Gastrodia elata Blume and has anti-oxidative stress effect. The potential protective activity of gastrodin in rat testicular ischemia-reperfusion injury model and underlying mechanism were explored. Male rats were randomized into three groups, including sham control, testicular ischemia-reperfusion injury, and testicular ischemia-reperfusion injury along with gastrodin injection (n = 20). Testicular ischemia-reperfusion injury group received 2-hr period of left testicular torsion (720° and counterclockwise) and 4-hr or 3-month period of testicular detorsion. At the onset of testicular detorsion, gastrodin-treated rats were given 100 mg kg-1 gastrodin by intra-peritoneal route. Following testicular detorsion, testicular tissues were collected for enzymatic activity analysis, oxidative stress evaluation, and histopathological examination. The ipsilateral testicular xanthine oxidase activity (source of reactive oxygen species production) and malondialdehyde level (a precise biomarker of reactive oxygen species) were significantly increased in testicular ischemia-reperfusion injury group versus sham control group, while testicular spermatogenic function was decreased. Also, gastrodin administration reduced xanthine oxidase activity and malondialdehyde level in ipsilateral testicular tissue, while improving testicular spermatogenic function. Consequently, it is suggested that gastrodin plays a protective role in testicular torsion/detorsion-induced ischemia/reperfusion injury through inhibiting xanthine oxidase activity to decrease reactive oxygen species formation.

Keywords

Gastrodin
Ischemia-reperfusion
Rat
Testicular torsion
Subjects
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Veterinary Research Forum
Volume 17, Issue 5
May 2026
Pages 315-320

  • Receive Date 28 June 2025
  • Revise Date 08 September 2025
  • Accept Date 11 October 2025

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