Veterinary Research Forum
Vet Res Forum

Cell SELEX-based DNA aptamer selection targeting Streptococcus agalactiae using high-throughput sequencing: toward multifunctional applications in aquaculture

Articles in Press

Document Type : Original Article

Authors

Dung Ho My Nguyen 1
Kamolwan Khianchaikhan 2
Apinya Longya 2
Nararat Laopichienpong 2
Thuan Duc Lao 3
Pimwarang Sukkarun 4

1 Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam

2 Department of research and development, Queen Saovabha Memorial Institute, Bangkok, Thailand

3 Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh City 722700, Vietnam

4 Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat, 80240, Thailand

10.30466/vrf.2025.2065775.4838
Abstract
Streptococcus agalactiae is a Gram-positive bacterial pathogen accountable for disease outbreaks in tilapia, resulting in substantial economic losses in aquaculture. In this study, a 10-round whole-cell Systemic Evolution of Ligands by Exponential Enrichment (SELEX) procedure was conducted to isolate and characterize single-stranded DNA (ssDNA) aptamers with specific binding affinity to S. agalactiae. The enriched ssDNA libraries were sequenced by high-throughput sequencing, yielding a total of 180,987 reads, of which 126,933 included unique aptamer sequences. The most frequent sequences were selected for further analysis. Among them, SA1 had the highest frequency, with 231 reads, whereas SA2 showed a lower number of 16 reads.
These findings underscore the validity of combining whole-cell SELEX, high-throughput sequencing, and aptamer-structure analysis to explore the interaction between aptamers and bacterial cell surfaces. The selected aptamers not only show promise for the detection of S. agalactiae but also serve as molecular probes for identifying surface-exposed proteins, studying host-pathogen interactions, and guiding targeted drug delivery in aquaculture-related applications.

Keywords

Aptamer
Streptococcus agalactiae
High-throughput sequencing
Whole-cell SELEX
Aquatic pathogens
Subjects
Veterinary Research Forum

Articles in Press, Accepted Manuscript
Available Online from 08 June 2026

  • Receive Date 16 July 2025
  • Revise Date 04 November 2025
  • Accept Date 15 November 2025

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