Veterinary Research Forum

Hematological, biochemical, and oxidative stress changes in lambs with atresia ani

Document Type : Original Article

Authors

Seyed Amir Hossein Hafezian 1
Abbas Raisi 2
Meysam Tehrani-Sharif 3
Khosro Safari Nikroo 4
Amir Zakian 2

1 DVM Graduate, Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran

3 Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Garmsar, Iran

4 Resident of Veterinary Surgery and Anesthesia, Department of Clinical Science, Faculty of Veterinary Medicine, Ferdowsi University, Mashhad, Iran

10.30466/vrf.2025.2065164.4822
Abstract
Anal atresia (AA) is a congenital anorectal malformation in neonatal lambs characterized by failure of the anal membrane to perforate, resulting in the absence of an anal opening. Early diagnosis and surgical correction are essential for improving survival and outcomes. This study evaluated hematobiochemical and oxidative stress parameters in neonatal lambs with non-segmental AA undergoing surgical intervention. Forty-five male lambs diagnosed with non-segmental AA were enrolled and stratified by time of presentation: within 24 hours of birth or 24-72 hours. An additional 45 healthy male lambs served as controls. Hematological and biochemical parameters, including white blood cell count, red blood cell count, hemoglobin, packed cell volume, total protein, C-reactive protein, superoxide dismutase, malondialdehyde, globulin, fibrinogen, and urea were measured at presentation and 3 days post-operatively. All AA lambs underwent surgical correction, with clinical follow-up for 30 days. Significant differences in hematobiochemical indices and oxidative stress markers were observed between AA lambs and healthy controls. Early surgical intervention (within 24 hours) was associated with significantly reduced levels of urea, fibrinogen, and C-reactive protein. It also resulted in lower malondialdehyde concentrations and higher superoxide dismutase activity, indicating decreased oxidative stress and inflammation compared with later intervention. These findings highlight the critical importance of early surgical intervention in neonatal lambs with anal atresia. Timely surgery improves hematological stability, mitigates oxidative damage, and supports better overall recovery.

Keywords

Anal atresia
Congenital anomaly
Lamb
Oxidative stress
Rectovaginal fistulae
Subjects
1.     Kavanya T, Rajendran R. Surgical management of congenital atresia ani (imperforate anus) in a madras red male lamb. Int J Curr Microbiol Appl Sci 2020; 9(7): 887-890.
2.     Ismail A, Mboma L, Ngotta V, et al. Anorectal malformation, associated with colon atresia and intestinal malrotation, a case report. Int J Surg Case Rep 2021; 84: 106159. doi: 10.1016/j.ijscr.2021.106159.
3.     Singh M, Mehra K. Imperforate Anus. In: StatPearls [Internet]. Updated 2023 Aug 28. Treasure Island (FL): StatPearls Publishing; 2024. Available at: https:// www.ncbi.nlm.nih.gov/books/NBK537154/. Accessed July 08, 2026.
4.     Azizi S, Mohammadi R, Mohammadpour I. Surgical repair and management of congenital intestinal atresia in 68 calves. Vet Surg 2010; 39(1): 115-120.
5.     Shakoor A, Muhammad SA, Younus M, et al. Surgical repair of congenital recto-vaginal fistula with atresia ani in a cow calf. Pak Vet J 2012; 32(2): 298-300.
6.     Mallesh P, Sampath K. Surgical management of congenital atresia ani (Imperforate anus) in a lamb: a case report. J Pharma Innov 2017; 6(7): 339-340.
7.     Fesseha H. Atresia ani et recti and its surgical correction in local begayit breed lamb. J Vet Res Adv 2020; 5(2): 5-8.
8.     Bodinga H, Abubakar N, Sidi S, et al. Surgical management of type 1 atresia ani in newborn lamb. Int J Res Sci Innov 2019; 6(9): 202-204.
9.     Trent AM, Ducharme NG, Fubini SL, et al. Surgery of the calf gastrointestinal system. In: Fubini SL, Ducharme NG (Eds). Farm animal surgery. 1st ed. St. Louis, USA: Saunders 2004; 461-484.
10. Chandrasekar T, Elango A, Kavanya T, et al. Surgical management of type II atresia ani (imperforate anus) in a Madras red male lamb: a case report. Int J Livest Res 2020; 10(5): 130-133.
11. Ghanem M, Yoshida C, Isobe N, et al. Atresia ani with diphallus and separate scrota in a calf: a case report. Theriogenology 2004; 61(7-8): 1205-1213.
12. Reduan MFH, Yahya SNC, Rajdi NZIM, et al. Anal reconstruction of type II atresia ani in a 3-day old Malin lamb. J Anim Health Prod 2020; 8(4): 221-223.
13. Daradka M. Surgical repair of atresia ani (imperforate anus) in newborn kids and lambs. JJAS 2013; 9(2): 193-200.
14. Rivera Maza S, Bishop RC, Austin SM, et al. Characteristics of meconium impaction/retention in newborn foals: from 2006 to 2024. Equine Vet Educ2025; 10.1111/eve.14109. doi: 10.1111/ eve.14109.
15. Li G, Wang S, Fan Z. Oxidative stress in intestinal ischemia-reperfusion. Front Med (Lausanne) 2022; 8: 750731. doi:10.3389/fmed.2021.750731.
16. Han X, Lang Z, Lv X, et al. Mitochondrial function in intestinal ischemia-reperfusion injury: mechanisms and therapeutic perspectives. Mol Biol Rep 2025; 52(1): 784. doi:10.1007/s11033-025-10894-5.
17. Wan Z, Zhang Y, Lv J, et al. Exosomes derived from bone marrow mesenchymal stem cells regulate pyroptosis via the miR-143-3p/myeloid differentiation factor 88 axis to ameliorate intestinal ischemia-reperfusion injury. Bioengineered 2023; 14(1): 2253414. doi:10.1080/21655979.2023.2253414.
18. Wang Z, Sun R, Wang G, et al. SIRT3-mediated deacetylation of PRDX3 alleviates mitochondrial oxidative damage and apoptosis induced by intestinal ischemia/reperfusion injury. Redox Biol 2020; 28: 101343. doi: 10.1016/j.redox.2019.101343.
19. Zhu Q, He G, Wang J, et al. Down-regulation of toll-like receptor 4 alleviates intestinal ischemia reperfusion injury and acute lung injury in mice. Oncotarget 2017; 8(8): 13678-13689.
20. Wang S, Zhang K, Huang Q, et al. TLR4 signalling in ischemia/reperfusion injury: a promising target for linking inflammation, oxidative stress and programmed cell death to improve organ transplantation outcomes. Mini Review. Front Immunol 2024;15: 1447060. doi: 10.3389/fimmu. 2024.1447060.
21. Ma Y, Zabell T, Creasy A, et al. Gut ischemia reperfusion injury induces lung inflammation via mesenteric lymph-mediated neutrophil activation. Original Research. Front Immunol 2020; 11: 586685. doi: 10.3389/fimmu.2020.586685.
22. Coşkun A, Aydoǧdu U, Altan S, et al. Hematologic, blood gas, cardiac biomarkers and serum biochemical parameters in calves with atresia coli and theirs relationship with prognosis. Acta Sci Vet 2017; 45(1): 6. doi: 10.22456/1679-9216.80726.
23. Salci H, Catik S, Ozdemir ES, et al. Peritoneal fluid analysis of the newborn calves with intestinal atresia - a clinical approach. Acta Sci Vet 2019; 47: 1701. doi: 10.22456/1679-9216.98189.
24. Bademkıran S, Icen H, Kurt D. Congenital recto vaginal fistula with atresia ani in a heifer: a case report. YYU Vet Fak Derg 2009; 20(1): 61-64.
25. Czerkiewicz I, Dreux S, Beckmezian A, et al. Biochemical amniotic fluid pattern for prenatal diagnosis of esophageal atresia. Pediatr Res 2011; 70(2): 199-202.
26. Muller C, Czerkiewicz I, Guimiot F, et al. Specific biochemical amniotic fluid pattern of fetal isolated esophageal atresia. Pediatr Res 2013; 74(5): 601-605.
27. Ryu J, Kang SG, Yun J, et al. Surgical repair of atresia ani with rectovaginal fistula in an African buffalo (Syncerus caffer). J Vet Clin 2018; 35(3): 111-113.
28. Kamalakar G, Devaratnam J, Brahmaji R, et al. Congenital atresia ani associated with recto-vaginal fistula in Ongole calf. J Livestock Sci 2015; 6: 80-84.
29. Grobe AC, Wells SM, Benavidez E, et al. Increased oxidative stress in lambs with increased pulmonary blood flow and pulmonary hypertension: role of NADPH oxidase and endothelial NO synthase. Am J Physiol Lung Cell Mol Physiol 2006; 290(6): L1069-L1077.
30. Aggarwal S, Gross C, Fineman JR, et al. Oxidative stress and the development of endothelial dysfunction in congenital heart disease with increased pulmonary blood flow: lessons from the neonatal lamb. Trends Cardiovasc Med 2010; 20(7): 238-246.
31. Williams DL. Congenital abnormalities in production animals. Vet Clin North Am Food Anim Pract 2010; 26(3): 477-486.
32. Aras-López R, Tovar JA, Martínez L. Possible role of increased oxidative stress in pulmonary hypertension in experimental diaphragmatic hernia. Pediatr Surg Int 2016; 32(2): 141-145.
33. Laforgia N, Di Mauro A, Favia Guarnieri G, et al. The role of oxidative stress in the pathomechanism of congenital malformations. Oxid Med Cell Longev 2018; 2018: 7404082. doi: 10.1155/2018/7404082.
34. Ozsurekci Y, Aykac K. Oxidative stress related diseases in newborns. Oxid Med Cell Longev 2016; 2016: 2768365. doi: 10.1155/2016/2768365.
35. Melek M, Demir H, Bilici S, et al. Oxidative stress and antioxidant enzyme activities in newborns with oesophageal atresia and their mothers. J Int Med Res 2012; 40(1): 249-257.
36. Asakawa T, Tanaka Y, Asagiri K, et al. Oxidative stress profile in the post-operative patients with biliary atresia. Pediatr Surg Int 2009; 25(1): 93-97.
37. Watanabe M, Borges FT, Pessoa EA, et al. Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury. Braz J Med Biol Res 2021; 54(11): e9941. doi: 10.1590/1414-431X2021e9941.
38. Wu L, Xiong X, Wu X, et al. Targeting oxidative stress and inflammation to prevent ischemia-reperfusion injury. Front Mol Neurosci 2020; 13: 28. doi: 10.3389/fnmol.2020.00028.
Veterinary Research Forum
Volume 17, Issue 7
July 2026
Pages 505-510

  • Receive Date 08 July 2025
  • Revise Date 29 August 2025
  • Accept Date 11 October 2025

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