1
Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41061, Korea
2
Biotoxtech, Co. Ltd., Cheongju, 29115, Korea
10.30466/vrf.2025.2065919.4839
Abstract
Spontaneous lymphoid neoplasms are common among aged C57BL/6 mice, which predominantly manifest as T cell lymphomas. However, primary B cell lymphoblastic lymphomas are rare and poorly characterized, especially in laboratory animals. This case report describes a primary B cell lymphoblastic lymphoma in the spleen of a 24-month-old male C57BL/6 mouse. Gross examination revealed an enlarged spleen with a homogenous, white, firm mass replacing the normal parenchyma. Histopathological analysis revealed that the splenic architecture was diffusely replaced by neoplastic lymphoid cells with high mitotic activity. Immunohistochemical staining confirmed the B cell origin of the neoplasm, demonstrating strong CD20 positivity and CD3 negativity. This case underscores the importance of detailed pathological evaluation in aged laboratory mice, especially to distinguish spontaneous lesions from treatment-induced effects in experimental studies. These findings contribute to the understanding of lymphoid neoplasms in laboratory animals, as well as provide valuable reference data for preclinical research in toxicology and oncology.
Lee,S , Sung,S , Sung,M , Kang,K , Park,J W , Park,S H , Kim,Y and Kim,D . (2026). Spontaneous primary B cell lymphoblastic lymphoma of the spleen of a 24-month-old male C57BL/6 mouse. (e737487). Veterinary Research Forum, (), e737487 doi: 10.30466/vrf.2025.2065919.4839
MLA
Lee,S , , Sung,S , , Sung,M , , Kang,K , , Park,J W , , Park,S H , , Kim,Y , and Kim,D . "Spontaneous primary B cell lymphoblastic lymphoma of the spleen of a 24-month-old male C57BL/6 mouse" .e737487 , Veterinary Research Forum, , , 2026, e737487. doi: 10.30466/vrf.2025.2065919.4839
HARVARD
Lee S, Sung S, Sung M, Kang K, Park J W, Park S H, Kim Y, Kim D. (2026). 'Spontaneous primary B cell lymphoblastic lymphoma of the spleen of a 24-month-old male C57BL/6 mouse', Veterinary Research Forum, (), e737487. doi: 10.30466/vrf.2025.2065919.4839
CHICAGO
S Lee, S Sung, M Sung, K Kang, J W Park, S H Park, Y Kim and D Kim, "Spontaneous primary B cell lymphoblastic lymphoma of the spleen of a 24-month-old male C57BL/6 mouse," Veterinary Research Forum, (2026): e737487, doi: 10.30466/vrf.2025.2065919.4839
VANCOUVER
Lee S, Sung S, Sung M, Kang K, Park J W, Park S H, Kim Y, Kim D. Spontaneous primary B cell lymphoblastic lymphoma of the spleen of a 24-month-old male C57BL/6 mouse. Vet Res Forum. 2026;():e737487. doi: 10.30466/vrf.2025.2065919.4839
