Veterinary Research Forum
Vet Res Forum

Effect of different surgical methods in the mouse embryo transfer: Electrosurgery versus cold surgical technique effects on repeated use of surrogate mothers, pregnancy rate and post-surgical behavior

Document Type : Original Article

Author

Navid Dadashpour Davachi

Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

https://doi.org/10.30466/vrf.2020.114953.2736
Abstract
Using the cold surgical technique (CST) is the most common practice to accomplish embryo transfer (ET). However, it can lead to uncontrolled bleeding and mortality in laboratory animals. Electrosurgery technique (EST) has provided the opportunity to prevent such complications. This study was aimed to evaluate CST versus EST in terms of repeated use of surrogate mothers, litter size, implantation rate and post-surgical behavior. Virgin female NMRI mice were allocated into two different surgical groups (n = 40): 1) CST-ET (control) and 2) EST-ET. Results showed that the first ET in EST-ET and CST-ET groups did not affect litter size, pregnancy rate and survival of surrogate mothers. Following the second and the third ETs, litter size was significantly affected through CST compared to EST, pregnancy rate and survival of surrogate mothers. Litter size, pregnancy rate and surrogate mothers survival rate did not show any significant reduction following the first and the second ETs in EST group. On the other hand, the third ET showed dramatic reduction for all aforementioned parameters regardless of the chosen surgical method for ET. Mice in EST-ET group did not show any significant change in their behavior indicating reduced well-being during the first 24 hr following the first, the second and the third ETs compared to CST-ET group. In conclusion, using EST for ET in mouse made it feasible to reuse surrogate mothers with minimum animal mortality; this could be pivotal with regard to reproductive and animal welfare aspects and research costs. Also, the results indicated that bleeding has severe diverse effects on ET efficiency.

Keywords

Cold surgery
Electrosurgery
Embryo transfer
Surrogate mother
  1.  Bermejo-Alvarez P, Park KE, Telugu BP. Utero-tubal embryo transfer and vasectomy in the mouse model. J Vis Exp 2014; (84): e51214.doi: 10.3791/51214.
  2. Kolbe T, Palme R, Touma C, et al. Repeated use of surrogate mothers for embryo transfer in the mouse. Biol Reprod 2012; 86(1): 1-6.
  3. Tarkowski AK. Experimental studies on regulation in the development of isolated blastomeres of mouse eggs. Acta Theriol 1959; 3(11): 191-287.
  4. Scott JE, Swanson EA, Cooley J, et al. Healing of canine skin incisions made with monopolar electrosurgery versus scalpel blade. Vet Surg 2017; 46(4): 520-529.
  5. Peneva M, Gjorgjeska A, Gjorgova ST, et al. Evaluation of pain following the use of scalpel versus electro-surgery for skin incisions in the facial regions. Pril (Makedon Akad Nauk Umet Odd Med Nauki) 2018; 39(2-3): 107-112.
  6. Aminimoghaddam S, Pahlevani R, Kazemi M. Electro-surgery and clinical applications of electrosurgical devices in gynecologic procedures. Med J Islam Repub Iran 2018; 32: 90.doi: 10.14196/mjiri.32.90.
  7. Al-Benna S. Albucasis, a tenth-century scholar, physician and surgeon: His role in the history of plastic and re-constructive surgery. Eur J Plast Surg 2011; 35: 379-387.
  8. Charoenkwan K, Iheozor-Ejiofor Z, Rerkasem K, et al. Scalpel versus electrosurgery for major abdominal incisions. Cochrane Database Syst Rev 2017; 6(6): CD005987. doi: 10.1002/14651858.CD005987.
  9. Dadashpour Davachi N, Zare Shahneh A, Kohram H, et al. Differential influence of ampullary and isthmic derived epithelial cells on zona pellucida hardening and in vitro fertilization in ovine. Reprod Biol 2016; 16(1): 61-69.
  10. Dadashpour Davachi N, Fallahi R, Dirandeh E, et al. Effects of co-incubation with conspecific ampulla oviductal epithelial cells and media composition on cryotolerance and developmental competence of in vitro matured sheep oocytes. Theriogenology 2018; 120: 10-15.
  11. Dadashpour Davachi N. Vasectomy in mouse model using electrosurgery machine. Arch Razi Inst 2019; 74(2): 191-195.
  12. Johnson CA. Reproductive disorders. In: Willard MD, Tvedten H, (Eds). Small animal clinical diagnosis by laboratory methods. 5th Saint Louis, USA: WB Saunders 2012; 294-303.
  13. Senger PL, Lose ED, Ulberg LC. Reduced blood supply to the uterus as a cause for early embryonic death in the mouse. J Exp Zool 1967; 165(3): 337-343.
  14. Père MC, Etienne M. Uterine blood flow in sows: effects of pregnancy stage and litter size. Reprod Nutr Dev 2000; 40(4): 369-382.
  15. Wagner LA, Menalled L, Goumeniouk AD, et al. Huntington Disease. In: McArthur RA, Borsini F, (Eds). Animal and translational models for CNS drug discovery. 1st San Diego, USA: Academic Press 2008; 207-266.
Veterinary Research Forum
Volume 12, Issue 4
Autumn 2021
Pages 467-471

  • Receive Date 27 September 2019
  • Revise Date 19 February 2020
  • Accept Date 09 May 2020

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