Document Type : Clinical Report
Private Veterinary Clinical Pathologist, Tehran, Iran
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Department of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization, Karaj, Iran
Dept of Internal diseases and clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Private Veterinary Practitioner, Tehran, Iran
A 2-year-old female Shih Tzu dog was submitted with the history of anorexia and depression for one week and no prior surgery. Fever and pale mucosa were noticed in physical examination. Microscopic examination of the Giemsa-stained blood smear disclosed large form of Babesia and single to four pear-shaped merozoites within erythrocytes (RBCs). Regenerative anemia characterized by a marked reticulocytosis, significant intra-vascular hemolysis, nucleated RBCs, left-shifted neutrophils, thrombocytopenia, azotemia, high serum creatinine and urea concentrations were recorded following hemato-biochemical analysis. Abundant bilirubin crystals and abnormal reddish color after centrifugation were observed in urinalysis. Molecular analysis was performed using specific primers for detection of Babesia canis. Diminazene aceturate, ciprofloxacin, ivermectin and phosphorus-vitamin B12 were prescribed and the clinical signs improved after four days. Two months follow-up showed no recurrence. Such studies would significantly contribute to the development of appropriate preventive strategies and successful treatment. This communication reports a clinical case of canine babesiosis caused by B. canis in a female Shih Tzu dog.
- Shabani B, Esmaeilnejad B, Tavassoli M, et al. Assessment of cholinesterase activity and hepatic biofunction in dogs naturally infected with Babesia gibsoni. Comp Clin Path 2020, 29: 1265-1269.
- Wang J, Liu J, Yang J, et al. Molecular detection and genetic diversity of Babesia canis canis in pet dogs in Henan Province, China. Parasitol Int 2019; 71: 37-40.
- Solano-Gallego L, Sainz Á, Roura X, et al. A review of canine babesiosis: The European perspective. Parasit Vectors 2016;9(1): doi:10.1186/s13071-016-1596-0.
- Boozer AL, Macintire DK. Canine babesiosis. Vet Clin North Am Small Anim Pract 2003; 33(4): 885-904, viii.
- Birkenheuer AJ, Levy MG, Breitschwerdt EB. Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and canis DNA in canine blood samples. J Clin Microbiol 2003; 41(9): 4172-4177.
- Furlanello T, Fiorio F, Caldin M, et al. Clinico-pathological findings in naturally occurring cases of babesiosis caused by large form Babesia from dogs of northeastern Italy. Vet Parasitol 2005; 134(1-2): 77-85.
- Weiss D, Tvedten H. The complete blood count, bone marrow examination, and blood banking: general comments and selected techniques. In: Willard M, Tvedten H (Eds). Small animal clinical diagnosis by laboratory methods. 5th Philadelphia, USA: Saunders 2011; 12-37.
- Niak A, Anwar M, Khatibi S. Canine babesiosis in Iran. Trop Anim Health Prod 1973; 5: 200-201.
- Razi jalali MH, Mosallanejad B, Avizeh R, et al. Babesia infection in urban and rural dogs in Ahvaz district, Southwest of Iran. Arch Razi Inst 2013; 68(1): 37-42.
- Helan A, Haddadzadeh H, Shirani D, et al. Histo-pathologic, hematologica and clinical study on canine babesiosis [Persian]. J Vet Res 2001; 56(3): 93-96.
- Bigdeli M, Mashhady Rafie S, Namavari MM, et al. Report of Theileria annulata and Babesia canis infections in dogs. Comp Clin Path 2012; 21(3): 375-377.
- Bigdeli M, Namavari MM. Evaluation of PCR assay using specific primers in diagnosis of canine ehrlichiosis and babesiosis: a study on herd and stray dogs in S J Altern Vet Med 2017; 1(1): 1-15.
- Hosseinzadeh Varjoy M, Ashrafi Helan J, Salehi N, et al. A survey on Babesia infection in dogs of urban and rural regions of Tabriz city, Iran [Persian]. Vet J (Pajouhesh & Sazandegi) 2016; 111: 56-63.
- Akhtardanesh B, Saberi M, Nurollahifard SR, et al. Molecular detection of Babesia spp. in tick-infested dogs in Southeastern Iran. J Dis Glob Health 2016; 8(2): 72-77.
- Kuleš J, Gotić J, Mrljak V, et al. Alteration of haemostatic parameters in uncomplicated canine babesiosis. Comp Immunol Microbiol Infect Dis 2017; 53: 1-6. doi: 10.1016/j.cimid.2017.06.001.