Veterinary Research Forum
Vet Res Forum

Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources

Document Type : Original Article

Authors

Mohadeseh Barazandeh 1, 2
Khashayar Shahin 1, 2
Abolghasem Hedayatkhah 3, 4
Majid Komijani 5
Mojtaba Mansoorianfar 6, 7

1 State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, China

2 Key Laboratory of Phage Research, International Phage Research Center, Jiangsu Academy of Agricultural Sciences, Nanjing, China

3 Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research and Utrecht University, Den Burg, The Netherlands

4 Department of Freshwater and Marine Ecology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands

5 Department of Biology, Faculty of Science, Arak University, Arak, Iran

6 Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran

7 Chinese Academy of Science, Suzhou Institute of Nano-Tech and Nano-Bionics, Suzhou, China

https://doi.org/10.30466/vrf.2020.110372.2618
Abstract
Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates of P. aeruginosa were collected from May to September 2018. Wastewater samples were used for isolation of lytic phage against XDR P. aeruginosa isolates. Host range, thermal and pH stability, adsorption rate, latent period, burst size and morphology of phage were determined following the standard protocols. Morphological characteristics of the phage revealed that it belonged to Podoviridae family and it was named vB-PaeP-007. Although the phage had a narrow host range, 47 out of 70 XDR isolates were susceptible to it. The adsorption rate, latent period and burst size of vB-PaeP-007 were approximately 89.80% in 8 min, 10 min and 93 phages per cell, respectively. Its lysis activity remained at a wide range of pH (4 up to 12) and temperature (– 20.00 up to 70.00 ˚C). Regarding the physiological features and host range of the vB-PaeP-007 phage, it could be a promising candidate for phage therapy and bio-controlling of infections from XDR isolates of P. aeruginosa in human and livestock storage centers.

Keywords

Bacteriophage
Extensively drug resistant
Phage therapy
Pseudomonas aeruginosa
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Veterinary Research Forum
Volume 12, Issue 4
Autumn 2021
Pages 401-407

  • Receive Date 27 June 2019
  • Revise Date 28 November 2019
  • Accept Date 08 January 2020

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