Veterinary Research Forum
Vet Res Forum

Molecular detection and analysis of virulence genes in multi-drug resistant Escherichia coli from infected broilers

Document Type : Short Communication

Authors

Kazi Faizul Azim 1
Saneya Risa Somana 1
Md Kamrul Hasan 2
Md Javed Foysal 2, 3
Md Hazrat Ali 2
Tanjia Afrin Chowdhury 1
Md Nazmul Hossain 1

1 Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh

2 Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh

3 School of Molecular and Life Sciences, Curtin University, Perth, Australia

https://doi.org/10.30466/vrf.2020.115921.2762
Abstract
Escherichia coli associated infections are major threats in poultry industry owing to severe economic losses each year. This study was conducted to identify E. coli isolates, to evaluate their antibiotic sensitivity and to find out their virulence patterns from infected broilers of Sylhet city in Bangladesh. Using polymerase chain reaction, a total 20 isolates were identified as E. coli from 11 chickens, exhibiting symptoms like colibacillosis and/or diarrhea. All isolates were positive for type-1 fimbrial adhesion (fimH), followed by putative avian hemolysin (hlyF) in 17 isolates; while none of the isolates was amplified with intimin (eaeA). Among 10 tested antibiotics, 100% of the isolates (n = 20) showed resistance to ampicillin, amoxicillin and tetra-cycline; but they were 100% sensitive to gentamicin. Organ specific correlations of antibiotic sensitivity were obtained among the isolates through principal component analysis (PCA) and Agglomerative Hierarchical Clustering (AHC). The 16S rRNA data of two multi-drug resistant isolates revealed closed clustering with clinical E. coli strains which could be indication of their zoonotic potential. In conclusion, the results depict higher prevalence of fimH and hlyF genes and drug resistance patterns of E. coli isolates from broilers in Sylhet city of Bangladesh.

Keywords

16s rRNA sequencing
Commercial broilers
Molecular detection
Multi-drug resistant E. coli
Virulence genes
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Veterinary Research Forum
Volume 12, Issue 4
Autumn 2021
Pages 505-510

  • Receive Date 20 October 2019
  • Revise Date 17 May 2020
  • Accept Date 01 June 2020

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