Veterinary Research Forum
Vet Res Forum

Gold nanoparticles improve the embryonic developmental competency of artificially activated mouse oocytes

Document Type : Original Article

Authors

Tahir Muhammad 1, 2
Muhammad Ameen Jamal 3
Muhammad Ashraf 3
Nosheen Zafar 4
Shamaila Shahzadi 4
Tahir Maqbool 2
Faheem Hadi 2
Riaz Amjad 1

1 Department of Theriogenology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan

2 Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan

3 Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, Pakistan

4 Department of Physics, Faculty of Natural Sciences, University of Engineering and Technology, Lahore, Pakistan

https://doi.org/10.30466/vrf.2020.119759.2829
Abstract
Currently, artificial oocyte activation has attracted wide attention in assisted reproduction due to extensive range of applications, particularly in somatic cell nuclear transfer and deriving pluripotent stem cell lines and it is the unique model to determine the role of paternal genome. Numbers of artificial activating agents have been used extensively to induce the oocytes activation; however, embryos developmental competency of artificially activated oocytes is still very low. In the present study, we determined the functional impact of strontium chloride supplementation with gold nanoparticles (AuNPs) in artificial oocytes activation and subsequent embryonic development. Oocytes were activated artificially in the culture medium containing 250 nM AuNPs with constant concentration of strontium chloride 10.00 mM. We found that adding 250 nM AuNPs with constant concentration of strontium chloride (10.00 mM for 3 hr) in culture medium improves the proportion of embryos reaching to the morula and blastocyst stages from 61.00% and 42.00% (controls) to 75.00% and 58.00% (250 nM AuNPs), respectively. In addition, foster mothers receiving AuNPs-treated embryos showed more implantation percentage and pregnancy rate relative to females received control embryos. Finally, embryos treated with 250 nM AuNPs concentration showed no toxic effect in term of blastocyst development. Collectively, our findings suggest the potential role of AuNPs in early embryonic development for mouse oocytes activated artificially and provide new insights in the field of animal biotechnology and assisted reproduction in humans.

Keywords

Embryonic development
Gold nanoparticles
Mice
Oocytes
Parthenogenesis
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Veterinary Research Forum
Volume 12, Issue 4
Autumn 2021
Pages 415-420

  • Receive Date 19 January 2020
  • Revise Date 30 June 2020
  • Accept Date 06 July 2020

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