Veterinary Research Forum
Vet Res Forum

Molecular identification and phylogenetic analysis of chronic bee paralysis virus in Iran

Document Type : Original Article

Authors

Mohammadreza Ghorani 1
Arash Ghalyanchi Langeroudi 1
Omid Madadgar 1
Mohammadreza Rezapanah 2
Sedigheh Nabian 3
Reza Khaltabadi Farahani 4
Hossein Maghsoudloo 4
Mohammad Forsi 4
Hamed Abdollahi 4
Hesameddin Akbarein 5

1 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Center of Excellence for Organic Agriculture and Department of Biological Control, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

3 Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

4 Iranian Veterinary Organization, Tehran, Iran

5 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract
Chronic bee paralysis virus (CBPV) is an unclassified polymorphic single-stranded RNA virus. Among the viruses infecting honeybees, CBPV is known to induce significant losses in honeybee colonies. In this study, a total number of eighty-nine suspected apiaries from four regions of Iran (including Mazandaran, Khorasan Razavi, Hormozgan, and Kurdistan) were sampled and submitted for molecular identification. Three positive samples were detected by RT-PCR. All positive samples were confirmed by sequencing. The phylogenetic tree which displays the molecular relationship between the viruses of different Iranian geographic regions and references isolates was constructed. The Iranian isolates formed two distinct phylogenetic groups (Group 1 and Group 2). The IR-CPV-GMG-1, IR-CPV-GMG-2, IR-CPV-GMG-4, and IR-CPV-GMG-6 formed Group 1 and IR-CPV-GMG-3, IR-CPV-GMG-5, and IR-CPV-GMG-7 were in Group 2 as a distinct group. Iranian isolates in group 1 were similar to European and East Asian CBPVs. This research was the first phylogenetic analysis of CBPV in Iran. Further researches are needed to study the other aspects of this virus-like genetic characteristics and pathogenesis in Iran.

Keywords

Chronic bee paralysis virus
Honeybee
Iran
Phylogenetic analysis
RT-PCR

Subjects

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Veterinary Research Forum
Volume 8, Issue 4
December 2017
Pages 287-292

  • Receive Date 31 January 2017
  • Revise Date 10 April 2017
  • Accept Date 17 April 2017

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