Veterinary Research Forum
Vet Res Forum

Effect of chlorpyrifos on sperm characteristics and testicular tissue changes in adult male rats

Document Type : Original Article

Authors

Mojtaba Babazadeh 1
Gholamreza Najafi 2

1 Graduate Student, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Abstract
Chlorpyrifos (CPF) is a broad spectrum organophosphate pesticide used for agricultural health purposes. Its principal mechanism of toxicity is the inhibition of acetylcholinesterase. The purpose of present study was to investigate the effects of CPF on testicular tissue and sperm parameters in male rats. Thirty-two healthy male rats were divided into two groups: a CPF-exposed group and a control-sham group. Control-sham group received corn oil (0.20 mL per day). The CPF was administered orally to male rats at 37 mg kg-1 BW for 45 days to evaluate the reproductive toxicity. In all rats, sampling for histological and sperm analyses was performed on days 5, 15, 30 and 45. The CPF caused a significant (p < 0.05) decrease in sperm count, viability and motility and increased immature sperms and DNA damage in sperm cells. Light microscopic analyses revealed increased arrested spermatogenesis, negative tubular differentiation and repopulation indexes and decreased Leydig cells number. These findings indicate that CPF has adverse effects on sperm cells and reproductive system of male rats.

Keywords

Chlorpyrifos
Histology
Rat
Sperm Testis

Subjects

  1. Xavier R, Rekha K, Bairy KL. Health perspective of pesticide exposure and dietary management. Mal J Nutr 2004; 10: 30-59.
  2. Peiris-John RJ, Wickremasinghe R. Impact of low-level exposure to organophosphate on human reproduction and survival. Trans R Soc Trop Med Hyg 2008; 102(3): 239-245.
  3. Muñoz-Quezada MT, Lucero BA, Iglesias VP, et al. Chronic exposure to organophosphate (OP) pesticides and neuropsychological functioning in farm workers: A review. Int J Occup Environ Health 2016; 22(1): 68-79.
  4. Eaton DL, Daroff RB, Autrup H, et al. Review of the toxicology of chlorpyrifos with an emphasis on human exposure and neurodevelopment. Crit Rev Toxicol 2008; 38: 1-125.
  5. Ambali SF, Akanbi DO, Oladipo OO, et al. Subchronic chlorpyrifos induced clinical, haematological and bio-chemical changes in Swiss albino mice: Protective effect of vitamin E. Int J Biol Med Res 2011; 2(2): 497-503.
  6. Uchendu C, Ambali SF, Ayo JO, et al. Subacute chlorpyrifos-induced alterations in serum lipids and some oxidative stress biomarkers in male Wistar rats: beneficial effect of acetyl-L-carnitine. Toxicol Environ Chem 2013; 95: 483-494.
  7. Joshi CS, Mathur R, Gulati N. Testicular toxicity of chlorpyrifos (an organophosphate Pesticide) in albino rats. Toxicol Ind Health 2007; 23(7): 439-444.
  8. Jalali AS, Hasanzadeh S, Malekinejad H. Crataegus monogyna aqueous extract ameliorates cyclo-phosphamide-induced toxicity in rat testis: Stereological evidences. Acta Med Iran2012;50(1): 1-8.
  9. Shetty G, Wilson G, Huhtaniemi I, et al. Gonadotropin releasing hormone analogs and testosterone inhibits the recovery of spermatogenesis in irradiated rats. Endocrinology 2000; 141: 1735-1745.
  10. Rezvanfar MA, Sadrkhanlou RA, Ahmadi A, et al. Protection of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA damage by an herbal source; evidence for role of free-radical toxic stress. Hum Exp Toxicol 2008; 27: 901-910.
  11. Suzuli N, Sofikitis N. Protective effects of antioxidants on testicular functions of varicocelized rats. Yonago Acta Med 1999; 42: 87-94.
  12. Hosseinchi M, Soltanalinejad F, Najafi G, et al. Effect of gibberellic acid on the quality of sperm and in vitro fertilization outcome in adult male rats. Vet Res Forum 2013 ; 4(4): 259–264.
  13. Zambrano E, Rodriguez-Gonzalez GL, Guzman C, et al. A maternal low protein diet during pregnancy and lactation in the rat impairs male reproductive development. J Physiol 2005; 563: 275-284.
  14. Wyrobek AJ, Gordon LA, Burkhart JG, et al. An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals. A report of the US environmental protection agency gene-tox program. Mutat Res 1983; 115: 1-72.
  15. Wong A, Chuan SS, Patton WC, et al. Addition of eosin to the aniline blue assay to enhance detection of immature sperm histones. Fertil Steril 2008; 90: 1999-2002.
  16. Tejada RI, Mitchell JC, Norman A, et al. A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence. Fertil Steril 1984; 42: 87-91.
  17. David JG. Toxicity of imidacloprid to Galendromus occidentalis, Neoseiulus fallacis and Amblyseius andersoni. Exp Applied Acarol 2004; 34: 275-281.
  18. Yurumez Y, Durukan P, Yavuz Y, et al. Acute organo-phosphate poisoning in university hospital emergency room patients. Intern Med 2007; 46: 965-969.
  19. Rotenberg M, Shefi M, Dany S, et al. Differentiation between organophosphate and carbamate poisoning. Clin Chim Acta 1995; 234: 11-21.
  20. Bo L. Therapeutic efficacies of different hemoperfusion frequencies in patients with organophosphate poisoning. Eur Rev Med Pharmacol Sci 2014; 18: 3521-3523.
  21. Deacon MM, Murray JS, Pilny MK, et al. Embryotoxicity and fetotoxicity of orally administered chlorpyrifos in mice. Toxico1 Appl Pharmacol 1980; 54: 31-40.
  22. Breslin WJ, Liberacki AB, Dittenber DA, et al. Evaluation of the developmental and reproductive toxicity of chlorpyrifos in the rat. Fundam Appl Toxico1 1996; 29: 119-130.
  23. Moser VC. Comparisons of the acute effects of cholinesterase inhibitors using a neurobehavioral screening battery in rats. Neurotoxicol and Teratol 1995; 17: 617-625.
  24. Corley RA, Calhoun LL, Dittenber DA, et al. Chlorpyrifos: A13-week nose-only vapor inhalation study in Fischer 344 rats. Fundam Appl Toxicol 1989; 13: 616-618.
  25. Sujatha R, Chitra K, Latchoumycandane C, et al. Effect of lindane on testicular antioxidant system and steroidogenic enzymes in adult rats. Asian J Androl 2001; 3: 135-138.
  26. Rahman MF, Mahboob M, Danadevi K, et al. Assessment of genotoxic effects of chlorpyriphos and acephate by the comet assay in mice leucocytes. Mutat Res 2002; 139: 516-547.
  27. Harfod AJ, Halloran K, Wright PFA. The effects of in vitro pesticide exposures on the phagocytic function of four native Australian freshwater fish. Aquatic Toxicol 2005; 75: 330-342.
  28. Verma RS, Mehta A, Srivastava N. In vivo chlorpyrifos induced oxidative stress: Attenuation by antioxidant vitamins. Pest Biochem Phys 2007; 88: 191-196.
  29. Ahmed NS, Mohamed AS, Abdel-Wahhab MA. Chlorpyrifos-induced oxidative stress and histological changes in retinas and kidney in rats: Protective role of ascorbic acid and alpha tocopherol. Pest Biochem Phys 2010; 98: 33-38.
  30. Thrasher JD, Madison R, Broughton A. Immunologic abnormalities in humans exposed to chlorpyrifos: Preliminary observations. Arch Environ Health 1993; 48: 89-93.
  31. Gupta SC, Siddique HR, Mathur N, et al. Adverse effect of organophosphate compounds dichlorvos and chlorpyrifos in the reproductive tissues of transgenic Drosophila melanogaster: 70 kDa heat shock protein as a marker of cellular damage. Toxicology 2007; 238: 1-14.
  32. Trasher JD, Heuser G, Broughton A. Immunological abnormalities in human chronically exposed to chlorpyrifos. Arch Environ Health 2002; 57: 181-187.
  33. Goel A, Dani V, Dhawan DK. Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos-induced toxicity. Chem Biol Interact 2005; 156: 131-140.
  34. Jett DA, Navoa RV. In vitro and in vivo effects of chlorpyrifos on glutathione peroxidase and catalase in developing rat brain. Neurotoxicology 2000; 21: 141-145.
  35. Ishii N, Senoo-Matsuda N, Miyake K, et al. Coenzyme Q10 can prolong C. elegans lifespan by lowering oxidative stress. Mech Ageing Dev 2004; 125: 41-46.
  36. Joshi CS, Mathur R, Gulati N. Testicular toxicity of chlorpyrifos (an organophosphate Pesticide) in albino rats. Toxicol Ind Health 2007; 23(7): 439-444.
  37. Dwivedi PD, Das M, Khanna SK. Role of cytochrome P450 in quinalphos toxicity: Effect on hepatic and brain antioxidant enzymes in rats. Food Chem Toxicol 1998; 36: 437- 444.
  38. de Lamirande E, Jiang H, Zini A, et al. Reactive oxygen species and sperm physiology. Rev Reprod 1997; 2(1): 48-54.
  39. Sikka SC. Relative impact of oxidative stress on male reproductive function. Curr Med Chem 2001; 8:
    851-862.
  40. Uzun FG, Kalender S, Durak D, et al. Malathion-induced testicular toxicity in male rats and the protective effect of vitamins C and E. Food Chem Toxicol 2009; 47: 1903-1908.
  41. Ferdinand N, Pierre W, Augustave K, et al. Effect of Dimethoate (an organophosphate insecticide) on the reproductive system and fertility of adult male rat. Am J Pharmacol Toxicol 2014; 9: 75-83.
  42. Farag AT, Ahmed F, Aswad E, et al. Assessment of reproductive toxicity of orally administered technical dimethoate in male mice. Reprod Toxicol 2007; 23: 232-238.
  43. Sayym F. Histopathological effects of dimethoate on testes of rats. Bull Environ Contam Toxicol 2007; 78: 479-484.
Veterinary Research Forum
Volume 8, Issue 4
December 2017
Pages 319-326

  • Receive Date 01 February 2017
  • Revise Date 16 November 2017
  • Accept Date 22 May 2017

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