Veterinary Research Forum
Vet Res Forum

Auto-transplantation of whole rat ovary in different transplantation sites

Document Type : Original Article

Authors

Somayeh Tavana 1
Mojtaba Rezazadeh Valojerdi 1, 2
Hussein Eimani 1, 3
Naeimeh Sadat Abtahi 1
Rouhollah Fathi 1

1 Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran

2 Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

3 Department of Anatomy, Faculty of Medicine, Baqiyatallah (AJ) University of Medical Sciences, Tehran, Iran

Abstract
This study was carried out to assess the different ovarian transplantation sites after short-time autografting. Female rats were randomized into five groups, with six rats in each group, including control (intact), cervical subcutaneous transplanted (CST), back subcutaneous transplanted (BST), subfascial transplanted (SFT) and intramuscular transplanted (IMT) groups. In all experimental groups, the right ovary was removed and transplanted into different sites. After three weeks, ovaries were removed for morphology assessment, follicular counting and the rates of corpus luteum (CL) and cyst formation. Transplanted ovaries in BST and SFT groups were full of cysts and did not have sufficient numbers of intact follicles and were excluded from experiments. In IMT and CST groups, re-anastomosis, follicular development and good homogeneity of the stromal tissue were seen. However, the difference in intact antral follicles between CST (7.92 ± 0.02%) and CST-Op (opposite ovary of CST group) (30.99 ± 0.03%) was significant as well as the difference between CST (7.92 ± 0.02%) and control (10.08 ± 0.01%) groups. In addition, the number of intact primordial follicles in the CST-Op (16.58 ± 0.02%) group was significantly less than that of the control (40.40 ± 0.03%) group. Interestingly, the number of CL was significantly increased in the CST-Op (11.71 ± 0.01%) and IMT-Op (9.16 ± 0.02%) groups compared to the control and experimental groups. Although both intramuscular and subcutaneous sites effectively preserved ovarian follicles after three weeks, cervical subcutaneous site was better suited for auto-transplantation in rat.

Keywords

Auto-transplantation
Rat
Transplantation site
Whole ovary

Subjects

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Veterinary Research Forum
Volume 8, Issue 4
December 2017
Pages 275-280

  • Receive Date 12 March 2017
  • Revise Date 01 August 2017
  • Accept Date 07 August 2017

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