Veterinary Research Forum
Vet Res Forum

Effect of experimental infectious bursal disease virus on clinical signs and pathogenesis of avian influenza virus H9N2 in turkey by real time PCR

Document Type : Original Article

Authors

Farhad Hashemzade 1, 2
Mansour Mayahi 3
Abdolhamid Shoshtary 4
Masoud Reza Seyfi- Abadshapuri 5
Masoud Ghorbanpoor 5

1 DVSc Graduate, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz

2 Chief of Poultry Diseases Specialized Hospital, Khoy, Iran

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Department of Avian Diseases, Razi Vaccine and serum research institute, Karaj, Iran

5 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

https://doi.org/10.30466/vrf.2018.75860.2013
Abstract
Infectious bursal disease virus (IBDV) in turkeys may result in immunosuppression, and inability of turkeys to resist nonpathogenic or less pathogenic organisms. A total number of 120 day-old commercial male turkeys were purchased and blood samples were collected from 20 day-old turkeys, remaining 100 were divided into four equal groups and kept in separated rooms. Groups 1 and 2 were infected with 104 CID50 of IBDV via intra-bursal route on day 1; Groups 1 and 3 were each infected with 106 EID50of AIV (H9N2) via the oculo-nasal routes on day 30. All groups were vaccinated against Newcastle disease vaccine (NDV). Detection of avian influenza virus H9N2 in trachea and cloaca swabs and in the tissues, was confirmed by Real-time polymerase chain reaction. Anti- NDV–AIV and anti-IBD titers were measured using HI and ELISA tests, respectively. The present study showed that infectious bursal disease changed the pathogenesis of (AIV) H9N2 by affecting AI virus replication and resulted in an increase shedding due to prolonged duration of sever clinical signs. The extent of shedding and virus replication need further study.

Keywords

Avian influenza
Infectious bursal disease virus
Real time PCR
Turkey

Subjects

  1.  

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Veterinary Research Forum
Volume 10, Issue 4
Autumn 2019
Pages 293-297

  • Receive Date 30 November 2017
  • Revise Date 02 September 2018
  • Accept Date 25 September 2018

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