Document Type : Clinical Report


1 Comprehensive Center for Precision Oncology, Medicine School of University of São Paulo, São Paulo, Brazil

2 Independent Veterinary Ophthalmologist Practitioner, Mogi das Cruzes, Brazil

3 Independent Veterinary Oncologist Practitioner, São Paulo, Brazil


Melanocytoma and malignant melanoma are the most typical eye tumors in dogs and cats. Due to the presence of melanin, the general appearance is often highly pigmented in a nodular formation with well-defined borders. Electrochemotherapy (ECT) is a therapy combining reversible electroporation and anti-neoplastic drugs to enhance their cytotoxic effects through increasing cellular uptake by the electroporated tumor cells. In this article, the use of adjuvant ECT for the treatment of canine ocular melanoma is reported. The pre-surgical exams (blood count, renal and liver functions, echocardiogram, and electrocardiogram) were within the normal range, as were the chest radiography and abdominal ultrasound without signs of metastases. On the day of the surgery, an excisional biopsy of the tumor was performed without safety margins by keratectomy associated with conjunctivectomy and adjuvant ECT in the surgical site and peri-tumoral region. The animal was followed for approximately 7 months and remained without evidence of tumor recurrence. A complete resolution of corneal opacification was also observed. To our knowledge, this is the first report of the use of ECT in an ocular neoplasm. From a comparative oncology perspective, this report opens the way for future therapeutic approaches in superficial ocular cancers in veterinary and human medicine.


Main Subjects

  1. Wang AL, Kern T. Melanocytic ophthalmic neoplasms of the domestic veterinary species: A review. Top Companion Anim Med 2015; 30(4): 148-157.
  2. Maggio F, Pizzirani S, Peña T, et al. Surgical treatment of epibulbar melanocytomas by complete excision and homologous corneoscleral grafting in dogs: 11 cases. Vet Ophthalmol 2012; 16(1): 56-64.
  3. Guerra Guimarães T, Menezes Cardoso K, Tralhão P, et al. Current therapeutics and future perspectives to ocular melanocytic neoplasms in dogs and cats. Bioengineering (Basel) 2021; 8(12): 225. doi: 10.3390/bioengineering8120225.
  4. Donaldson D, Sansom J, Scase T, et al. C. Canine limbal melanoma: 30 cases (1992-2004). Part 1. Signalment, clinical and histological features and pedigree analysis. Vet Ophthalmol 2006; 9(2): 115-119.
  5. McMullen RJ, Clode AB, Pandiri AK, et al. Epibulbar melanoma in a foal. Vet Ophthalmol 2008; Suppl 1(Suppl 1): 44-50.
  6. Branisteanu DC, Bogdanici CM, Branisteanu DE, et al. Uveal melanoma diagnosis and current treatment options (review). Exp Ther Med 2021; 22(6). 1428. doi: 10.3892/etm.2021.10863.
  7. Luz JCDSD, Antunes F, Clavijo-Salomon MA, et al. Clinical applications and immunological aspects of electroporation-based therapies. Vaccines (Basel) 2021; 9(7): 727. doi: 10.3390/vaccines9070727.
  8. Marty M, Sersa G, Garbay JR, et al. Electro-chemotherapy - an easy, highly effective and safe treatment of cutaneous and subcutaneous metastases: Results of ESOPE (European standard operating procedures of electrochemotherapy) study. EJC Suppl 2006; 4(11): 3-13.
  9. Ivorra A, Al-Sakere B, Rubinsky B, et al. Use of conductive gels for electric field homogenization increases the antitumor efficacy of electroporation therapies. Phys Med Biol 2008; 53(22): 6605-6618.
  10. Suzuki DO, Marques CM, Rangel MM. Conductive gel increases the small tumor treatment with electro-chemotherapy using needle electrodes. Artif Organs 2015; 40(7): 705-711.
  11. Fiorentzis M, Viestenz A, Siebolts U, et al. The potential use of electrochemotherapy in the treatment of uveal melanoma: In Vitro results in 3D tumor cultures and In Vivo results in a chick embryo model. Cancers (Basel) 2019; 11(9): 1344. doi: 10.3390/cancers11091344
  12. Salwa SP, Bourke MG, Forde PF, et al. Electro-chemotherapy for the treatment of ocular basal cell carcinoma; a novel adjunct in the disease management. J Plast Reconstr Aesthet Surg 2014; 67(3): 403-406.
  13. Kis EG, Baltás E, Ócsai H, et al. Electrochemotherapy in the treatment of locally advanced or recurrent eyelid-periocular basal cell carcinomas. Sci Rep 2019; 9(1): 4285. doi: 10.1038/s41598-019-41026-2.