Veterinary Research Forum
Vet Res Forum

Immuno-chromatography based rapid detection of canine distemper in dogs and its comparison with RT-PCR

Document Type : Original Article

Authors

Jharna Koul 1
Divya Agnihotri 2
Ricky Jhambh 1
Tarun Kumar 2
Gaurav Charaya 1

1 Department of Veterinary Medicine, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

2 Department of Veterinary Clinical Complex, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

https://doi.org/10.30466/vrf.2024.2030571.4301
Abstract
The present study was planned to confirm the clinical diagnosis of canine distemper in dogs with reverse transcriptase polymerase chain reaction (RT-PCR) and its comparison with lateral flow assay based immuno-chromatographic (IC) technique. Fifty clients owned dogs having clinical signs suggestive of respiratory, skin or nervous form of canine distemper were included in the study. An immuno-chromatography-based test was applied using serum to screen each of the suspected dog. In addition to serum, nasal discharges of 15 cases and ocular discharges of 10 samples were used to diagnose canine distemper. Screening with IC kit revealed 72.00% serum samples positive, 66.66% ocular and 50.00% nasal samples were found positive for antigen. The RT-PCR targeting N gene of canine distemper virus was used for the molecular diagnosis of canine distemper. Out of 50 blood samples tested with RT-PCR assay, 38 (76.00%) samples were positive showing characteristics band of 287bp. Statistical comparison of IC Kit (serum samples) results with RT-PCR results comparison showed that IC kit was 52.00% accurate with 36.84% sensitivity, 100% specificity, positive predictive value of 93.33% and negative predictive value of 31.43%. In the current study it was observed that the IC test was rapid, quick and specific but was found to be less sensitive compared to RT-PCR.

Keywords

Canine distemper
Immuno-chromatography
RT-PCR
Sensitivity
Specificity
Subjects
  1. Litster AL, Pressler B, Volpe A, et al. Accuracy of a point-of-care ELISA test kit for predicting the presence of protective canine parvovirus and canine distemper virus antibody concentrations in dogs. Vet J2012; 193(2): 363-366.
  2. Rendon-Marin S, da Fontoura Budaszewski R, Canal WC, et al. Tropism and molecular pathogenesis of canine distemper virus. Virol J 2019; 16(1): 30. doi: 10.1186/s12985-019-1136-6.
  3. Awad RA. Rapid approaches for diagnosis of canine distemper virus in live and dead dogs in Egypt. Egypt J Vet Sci 2019; 50(1): 47-56.
  4. Kim YH, Cho KW, Youn HY, et al. Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested J Vet Sci2001; 2(1): 59-63.
  5. Amude AM, Alfieri AA, Alfieri AF. Clinicopathological findings in dogs with distemper encephalomyelitis presented without characteristic signs of the disease. Res Vet Sci 2007; 82(3): 416-422.
  6. Frisk AL, König M, Moritz A, et al. Detection of canine distemper virus nucleoprotein RNA by RT-PCR using serum, whole blood and cerebrospinal fluid from dogs with distemper. J Clin Microbiol 1999; 37(11): 3634-3643.
  7. Desai D, Kalyani I, Solanki JA, et al. Serological and nucleocapsid gene based molecular characterization of canine distemper virus (CDV) isolated from dogs of southern Gujarat, India. Indian J AnimRes2020; 55(10): 1224-1232.
  8. An DJ, Kim TY, Song DS, et al. An immuno-chromatography assay for rapid antemortem diagnosis of dogs suspected to have canine distemper. J Virol Methods 2008; 147(2): 244-249.
  9. Yang DK, Kim HH, Lee J, et al. Evaluation of commercial immunochromatographic test kits for the detection of canine distemper virus. J Bacteriol Virol 2020; 50(2): 117-123.
  10. Saito TB, Alfieri AA, Wosiacki SR, et al. Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis. Res Vet Sci 2006; 80(1): 116-119.
  11. Swati, Dipak D, Ramneek. Detection of local isolates of canine distemper virus by reverse-transcription polymerase chain reaction. Int J Curr Microbiol App Sci 2016; 5(5): 757-766.
  12. Ashmi JM, Thangavelu A, Senthilkumar, TMA, et al. Molecular characterization of canine distemper virus from Tamil Nadu, India. Indian J Anim Sci 2017; 87(9): 1062-1067.
  13. Pawar RM, Raj GD, Gopinath VP, et al. Isolation and molecular characterization of canine distemper virus from India. Trop Anim Health Prod 2011; 43(8): 1617-1622.
  14. Silva MMO, Castro TX, Costa EM, et al. Comparison of three laboratorial tests for diagnosis of canine parvovirus infection. Arq Bras Med Vet Zootec 2013; 65(1): 149-152.
  15. Costa VGD, Saivish MV, Rodrigues RL, et al. Molecular and serological surveys of canine distemper virus: a meta-analysis of cross-sectional studies. PLoS One 2019; 14(5): e0217594. doi: 10.1371/journal.pone. 0217594.
  16. Gebara CMS, Wosiacki SR, Negrão FJ, et al. Detection of canine distemper virus nucleoprotein gene by RT-PCR in urine of dogs with distemper clinical signs [Portuguese]. Arq Bras Med Vet Zootec 2004; 56(4): 480-487.
  17. Alcalde R, Kogika MM, Fortunato VAB, et al. Canine distemper virus: detection of viral RNA by nested RT-PCR in dogs with clinical diagnosis. Braz J Vet Res Anim Sci 2013; 50(1): 74-76.
  18. Tavakoli Zaniani A, Mokhtari A, Esmailnejad A. Molecular and immunological investigation of canine distemper virus (CDV) and its co- infection with canine parainfluenza virus type 2. Iran J Med Microbiol 2021; 15(2): 212-226.
Veterinary Research Forum
Volume 16, Issue 5
May 2025
Pages 261-265

  • Receive Date 24 June 2024
  • Revise Date 21 September 2024
  • Accept Date 05 November 2024

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