Veterinary Research Forum
Vet Res Forum

First report of histopathological and molecular characterizations of bovine herpesvirus-1 from outbreak at dairy farm in India

Document Type : Clinical Report

Authors

Amit Kumar Verma
Vikas Jaiswal
Arbind Singh
Ramakant .
Vishnu Kumar Rai

Department of Veterinary Medicine, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, India

https://doi.org/10.30466/vrf.2024.2028036.4260
Abstract
Infectious bovine rhinotracheitis, caused by bovine herpesvirus-1 (BHV-1) is a highly contagious viral disease affecting bovines, and clinically characterized by pyrexia, inappetence, respiratory distress, dyspnoea, conjunctivitis, nasal discharge, and sometimes abortions. In the present study, buffalo dairy farm having high mortality was investigated. The buffaloes were suffering from high rectal temperature, conjunctivitis, severe respiratory distress, and nasal discharge. Tissue samples from upper respiratory tract were collected aseptically following post-mortem examination of died buffaloes. Tracheal tissue samples were then processed for histopathological examination and DNA isolation. The presence of BHV-1 in the tissue samples was confirmed by nested polymerase chain reaction using glycoprotein B gene primers. The present study reported for the first time the clinical signs, post-mortem lesions, histopathological evidence, and detection of DNA of BHV-1 glycoprotein B gene through nested polymerase chain reaction assay during an active outbreak in buffaloes in India. The findings of this study are crucial for improving the diagnosis of BHV-1 and ultimately reducing financial losses within dairy industry.

Keywords

Bovine herpesvirus-1
Buffalo
India
Polymerase chain reaction
Subjects
  1. Wyler R, Engles M, Schwyzer M. Infectious bovine rhinotracheitis/vulvovaginitis (BHV-1). In: Wittmann G (Ed). Herpesvirus diseases of cattle, horses and pigs. Boston, USA: Kluwer Academic Publishers 1989; 1-72.
  2. Chandranaik BM, Shivaraj C, Sanjeev K. et al. Isolation of BHV-1 from bovine semen and application of real time PCR for diagnosis of IBR/IPV from clinical samples. Vet Arh2010; 80(4): 467-475.
  3. Biswas S, Bandyopadhyay S, Dimri U, et al. Bovine herpesvirus-1 (BHV-1) - a re-emerging concern in livestock: a revisit to its biology, epidemiology, diagnosis, and prophylaxis. Vet Q 2013; 33(2): 68-81.
  4. Yesilbag K, Bilge-dagalap S, Okur-Gumusova S, et al. Studies on herpesvirus infections of goats in Turkey: prevalence of antibodies to bovine herpesvirus 1. Revue Méd Vét2003; 154(12): 772-774.
  5. Rajkhowa S, Rajkhowa C, Rahman H, et al. Sero-prevalence of infectious bovine rhinotracheitis in mithun (Bos frontalis) in India. Rev Sci Tech 2004; 23(3): 821-829.
  6. Yazıcı Z, Albayrak H, Ozan E, et al. Serological status of bovine herpes virus type 1 in cattle in small scale private farms in the Central Black Sea Region, Turkey. Pak Vet J 2015; 35(1): 101-102.
  7. Muylkens B, Thiry J, Kirten P, et al. Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis. Vet Res 2007; 38(2): 181-209.
  8. Nandi S, Kumar M, Yadav V, et al Serological evidences of bovine herpesvirus-1 infection in bovines of organized farms in India. Transbound Emerg Dis 2011; 58(2): 105-109.
  9. Singh R, Verma AK, Sharma B, et al Detection of bovine herpesvirus-l (BHV-l) infection in cattle by antigen detection ELISA and multiplex PCR. Adv Anim Vet Sci 2013; 1(3S): 12-16.
  10. Verma AK, Kumar A, Sahzad PN, et al. Sero-prevalence of infectious bovine rhinotracheitis in dairy animals with reproductive disorders in Uttar Pradesh, India. Pak J Biol Sci 2014; 17(5): 720-724.
  11. Luna LG. Manual of histologic staining methods of the Armed Forces Institute of Pathology. 3rd New York, USA: McGraw-Hill 1968; 32-47.
  12. Rocha MA, Barbosa EF, Guedes RM, et al. Detection of BHV-1 in a naturally infected bovine fetus by a nested PCR assay. Vet Res Commun 1999; 23(2): 133-141.
  13. Raaperi K, Bougeard S, Aleksejev A. et al. Association of herd BHV-1 seroprevalence with respiratory disease in young stock in Estonian dairy cattle. Res Vet Sci 2012; 93(2): 641-648.
  14. López A, Martinson SA. Respiratory System, Mediastinum, and Pleurae. Pathologic Basis Vet Dis 2017; 471-560.e1. doi: 10.1016/B978-0-323-35775-3.00009-6.
  15. Mahajan V, Banga HS, Deka D. Comparison of diagnostic tests for diagnosis of infectious bovine rhinotracheitis in natural cases of bovine abortion. J Comp Path 2013; 149(4): 391-401.
Veterinary Research Forum
Volume 16, Issue 5
May 2025
Pages 301-304

  • Receive Date 07 May 2024
  • Revise Date 26 December 2024
  • Accept Date 29 December 2024

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