Veterinary Research Forum
Vet Res Forum

Effect of different ratios of male-to-female in broilers on performance and nutrients digestibility

Document Type : Original Article

Authors

Motaleb Ebrahimi 1
Mohsen Daneshyar 1
Hamed Ahmadi 2
Sina Payvastegan 1
Mohammad Afrouziyeh 3
Gholamreza Najafi 4

1 Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran

2 Department of Poultry Science, Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran

3 Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada

4 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2025.2043289.4494
Abstract
Male and female broiler chickens differ in performance and this will cause unwanted experimental errors in research. For this reason, single-sex or mixed-sex broilers are used in most studies. This study aimed to assess the performance differences between groups of chickens with varying male/female ratios to determine how sex ratio can affect performance criteria. Birds (N = 550) were separated by sex and placed in 11 groups (pens) according to the male/female ratios, including group 1 (10 males + 0 female), group 2 (9 males + 1 female), group 3 (8 males + 2 females), group 4 (7 males + 3 females), group 5 (6 males + 4 females), group 6 (5 males + 5 females), group 7 (4 males + 6 females), group 8 (3 males + 7 females), group 9 (2 males + 8 females), group 10 (1 male + 9 females), and group 11 (0 male + 10 females). The results showed that male broiler chickens had higher feed intake and body weight gain than female broiler chickens, but the feed conversion ratio was not affected by gender. The digestibility of phosphorus, bone strength, bone density, bone calcium and phosphorus, pH, and redness and water holding capacity of meat were higher in male broilers. The dripping loss was higher in female broilers. This study showed that male and female broiler chickens differed in most of the parameters examined in the research, and the use of separate breeding affected the research results. 

Keywords

Bone density
Broiler chickens
Gender
Meat color

Subjects

1.     Henry MH, Burke WH. Sexual dimorphism in broiler chick embryos and embryonic muscle development in late incubation. Poult Sci 1998; 77(5): 728-736.
2.     May JD, Lott BD. Relating weight gain and feed: gain of male and female broilers to rearing temperature. Poult Sci 2001; 80(5): 581-584.
3.     England A, Gharib-Naseri K, Kheravii SK, et al. Influence of sex and rearing method on performance and flock uniformity in broilers-implications for research settings. Anim Nutr 2023; 12: 276-283.
4.     Gous RM, Moran ET Jr, Stilborn HR, et al. Evaluation of the parameters needed to describe the overall growth, the chemical growth, and the growth of feathers and breast muscles of broilers. Poult Sci 1999; 78(6): 812-821.
5.     Otsuka M, Miyashita O, Shibata M, et al. A novel method for sexing day-old chicks using endoscope system. Poult Sci 2016; 95(11): 2685-2689.
6.     Phelps P, Bhutada A, Bryan S, et al. Automated identification of male layer chicks prior to hatch. Worlds Poult Sci J 2003; 59(1): 33-38.
7.     Doran TJ, Morris KR, Wise TG, et al. Sex selection in layer chickens. Anim Prod Sci 2017; 58(3): 476-480.
8.     England AD, Kheravii SK, Musigwa S, et al. Sexing chickens (Gallus gallus domesticus) with high-resolution melting analysis using feather crude DNA. Poult Sci 2021; 100(3):100924. doi: 10.1016/j.psj.2020.12.022.
9.     Lamoreux WF, Proudfoot FG. Effects on body weight and feed conversion of broiler chickens from three commercial crosses reared with the sexes separated or intermingled. Can J Anim Sci 1969; 49(1): 23-28.
10. Albuquerque R, Marchetti LK, Fagundes AC, et al. Effect of different population densities and sex on performance and uniformity in broilers (2006) [Portuguese]. Braz J Vet Res Anim Sci 2006; 43(5): 581-587.
11. Da Costa MJ, Colson G, Frost TJ, et al. Straight-run vs. sex separate rearing for two broiler genetic lines Part 2: economic analysis and processing advantages. Poult Sci 2017; 96(7): 2127-2136.
12. Aviagen. Ross 308 parent stock: nutrition specifications. Available at: https://aviagen.com/ assets/Tech_Center /Ross_PS/Ross308-EuropeanParentStock-Nutrition Specifications-2021-EN.pdf. Accessed: 18 September, 2025.
13. Osunbami OT, Walk CL, Adeola O. Digestible calcium equivalency of phytase and nutrient utilization of broiler chickens fed graded levels of limestone or phytase during the starter phase. Poult Sci 2024; 103(2): 103360. doi: 10.1016/j.psj.2023.103360.
14. Haetinger VS, Adeola O. Comparison of different protein sources on the phosphorus digestibility of soybean meal for broiler chickens determined using the regression method. Poult Sci 2024; 103(2): 103327. doi: 10.1016/j.psj.2023.103327.
15. Fenton TW, Fenton M. An improved procedure for the determination of chromic oxide in feed and feces. Can J Anim Sci 1979; 59(3): 631-634.
16. Short FJ, Gorton P, Wiseman J, et al. Determination of titanium dioxide added as an inert marker in chicken digestibility studies. Anim Feed Sci Technol 1996; 59(4): 215-221.
17. Kong C, Adeola O. Evaluation of amino acid and energy utilization in feedstuff for swine and poultry diets. Asian-Australas J Anim Sci 2014; 27(7): 917-925.
18. Pedersen IJ, Tahamtani FM, Forkman B, et al. Effects of environmental enrichment on health and bone characteristics of fast growing broiler chickens. Poult Sci 2020; 99(4): 1946-1955.
19. Liu SB, Liao XD, Lu L, et al. Dietary non-phytate phosphorus requirement of broilers fed a conventional corn-soybean meal diet from 1 to 21 d of age. Poult Sci 2017; 96(1): 151-159.
20. AOAC. Official methods of analysis. 18th ed. Washington DC, USA: Association of Official Analytical Chemists, 2006.
21. Dai SF, Gao F, Xu XL, et al. Effects of dietary glutamine and gamma-aminobutyric acid on meat colour, pH, composition, and water-holding characteristic in broilers under cyclic heat stress. Br Poult Sci 2012; 53(4): 471-481.
22. Christensen LB. Drip loss sampling in porcine m. longissimus dorsi. Meat Sci 2003; 63(4): 469-477.
23. Aziz Mesgari Z, Daneshyar M, Aghazadeh A. Effects of betaine on performance, some blood indices, antioxidant status and internal organ weights of broiler chickens under heat stress [Persian]. J Vet Res 2017; 72(3): 297-304.
24. Qiao M, Fletcher DL, Northcutt JK, et al. The relationship between raw broiler breast meat color and composition. Poult Sci 2002; 81(3): 422-427.
25. López KP, Schilling MW, Corzo A. Broiler genetic strain and sex effects on meat characteristics. Poult Sci 2011; 90(5): 1105-1111.
26. Madilindi MA, Mokobane A, Letwaba PB, et al. Effects of sex and stocking density on the performance of broiler chickens in a sub-tropical environment. S Afr J Anim Sci 2018; 48(3): 459-468.
27. Goo D, Kim JH, Choi HS, et al. Effect of stocking density and sex on growth performance, meat quality, and intestinal barrier function in broiler chickens. Poult Sci 2019; 98(3): 1153-1160.
28. Aggrey SE, Karnuah AB, Sebastian B, et al. Genetic properties of feed efficiency parameters in meat-type chickens. Genet Sel Evol 2010; 42(1): 25. doi: 10.1186/1297-9686-42-25.
29. Zerehdaran S, Vereijken AL, van Arendonk JA, et al. Effect of age and housing system on genetic para-meters for broiler carcass traits. Poult Sci 2005; 84(6): 833-838.
30. England AD, Gharib-Naseri K, Kheravii SK, et al. Rearing broilers as mixed or single-sex: relevance to performance, coefficient of variation, and flock uniformity. Poult Sci 2022; 101(12): 102176. doi: 10.1016/j.psj.2022.102176.
31. Singh V, Singh U, Sethi AP, et al. Gender effect on growth performance and nutrient digestibility in (IBL-80) broilers. Haryana Vet 2020; 59(1): 6-9.
32. Muñoz JA, Suckeveris D, Demuner LF, et al. Effects of nutritional levels on performance, carcass characteristics and nutrient digestibility of sexed broilers. Rev Bras Cienc Avic 2018; 20(1): 53-62.
33. Ten Doeschate RA, Scheele CW, Schreurs VV, et al. Digestibility studies in broiler chickens: influence of genotype, age, sex and method of determination. Br Poult Sci 1993; 34(1): 131-146.
34. Lee KC, Kil DY, Sul WJ. Cecal microbiome divergence of broiler chickens by sex and body weight. J Microbiol 2017; 55(12): 939-945.
35. Ziaei N, Guy JH, Edwards SA, et al. Effect of gender on factors affecting excreta dry matter content of broiler chickens. J Appl Poult Res 2007; 16(2): 226-233.
36. Tatara MR, Sierant-Rozmiej N, Krupski W, et al. Quantitative computed tomography for the assessment of mineralization of the femur and tibia in turkeys. Med Weter 2005; 61(2): 225-228.
37. Charuta A. Evaluation of densitometric and geometric parameters of the femur in 14-month-old ostriches depending on sex with the use of computed tomography. J Vet Res 2013; 57(2): 287-291.
38. Alkhtib A, Sanni CO, Burton E, et al. What is “normal”? Morphology and mineralization of tibias from healthy, on farm broilers. J Appl Poult Res 2021; 30(3): 100190. doi: 10.1016/j.japr.2021.100190.
39. Müsse J, Louton H, Spindler B, et al. Sexual dimorphism in bone quality and performance of conventional broilers at different growth phases. Agriculture 2022; 12(8): 1109. doi: 10.3390/agriculture12081109.
40. Rath NC, Huff WE, Balog JM, et al. Effect of gonadal steroids on bone and other physiological parameters of male broiler chickens. Poult Sci 1996; 75(4): 556-562.
41. Torres TR, Ludke M do CM M, Ludke JV, et al. Performance of broilers fed during 21 days on mash or pellet diets containing whole or ground pearl millet grain. Rev Bras Cienc Avic 2013; 15(4): 371-378.
42. Fletcher DL. Poultry meat quality. Worlds Poult Sci J 2002; 58(2): 131-145.
43. Hailemariam A, Esatu W, Abegaz S, et al. Effect of genotype and sex on breast meat quality characteristics of different chickens. J Agric Food Res 2022; 10: 100423. doi: 10.1016/j.jafr.2022.100423.
44. Schneider BL, Renema RA, Betti M, et al. Effect of holding temperature, shackling, sex, and age on broiler breast meat quality. Poult Sci 2012; 91(2): 468-477.
45. Barbut S, Leishman EM. Quality and processability of modern poultry meat. Animals (Basel) 2022; 12(20): 2766. doi: 10.3390/ani12202766.
46. Jaturasitha S, Srikanchai T, Kreuzer M, et al. Differences in carcass and meat characteristics between chicken indigenous to northern Thailand (Black-boned and Thai native) and imported extensive breeds (Bresse and Rhode Island Red). Poult Sci 2008; 87(1): 160-169.
47. Gálvez F, Domínguez R, Pateiro M, et al. Effect of gender on breast and thigh turkey meat quality. Br Poult Sci 2018; 59(4): 408-415.
48. Özbek M, Petek M, Ardıçlı S. Physical quality characteristics of breast and leg meat of slow-and fast-growing broilers raised in different housing systems. Arch Anim Breed 2020; 63(2): 337-344.
Veterinary Research Forum
Volume 16, Issue 11
November 2025
Pages 667-674

  • Receive Date 12 October 2024
  • Revise Date 19 February 2025
  • Accept Date 19 February 2025

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