Embryology
Ahmad reza raji; shadi Hashemnia; Ahmad ail Mohammadpour; Abolghasem Nabipour; zohre saddatfar
Articles in Press, Accepted Manuscript, Available Online from 05 May 2024
Abstract
Ostrich (Struthio camelus) is an important wild species highlighted in national and international livestock. This research was conducted to analyze the development of the ostrich respiratory system in fetal and embryonic stages. A total of 50 fertile ostrich eggs were collected from commercial farms. ...
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Ostrich (Struthio camelus) is an important wild species highlighted in national and international livestock. This research was conducted to analyze the development of the ostrich respiratory system in fetal and embryonic stages. A total of 50 fertile ostrich eggs were collected from commercial farms. They were incubated at 36–37°C and 25 ± 2% humidity for 40 days. Sections were taken in 13, 22, 26, 30, 36, and 42 days of incubation from the lung and the cranial, middle, and caudal parts of the neck after decapitation of ostrich embryos and blood drainage. After fixation, processing, blocking, and sectioning, all samples were stained by hematoxylin and eosin, Alcian blue (AB), Van Gieson, and Periodic acid-Schiff (PAS) techniques.It was concluded that the trachea in the 13-day-old embryo (E13) and goblet cells (PAS-positive and AB-positive) had incomplete rings of hyaline cartilages and differentiation of mesenchymal to the loose connective tissue.Bronchial stage of the lung in the E22, pseudo-glandular stage in the E26, and parabrachial and air capillary stage in the E30 were also observed. The information obtained here will be useful for diagnosing the pathologies affecting this vital system and results in improving industrial breeding management.
Entekhab Hameed Abed AL-Shuwaili; Abolghasem Nabipour; Azam Hosseini; Hesam Dehghani
Volume 13, Issue 4 , December 2022, , Pages 521-527
Abstract
Stage X is one of the formation stages in birds at which the blastoderm area is distinguished by two areas of area pellucida being responsible for formation of embryonic tissues and primordial germ cells, and area opaca forming the extra-embryonic tissues. Primordial germ cells are multi-potent stem ...
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Stage X is one of the formation stages in birds at which the blastoderm area is distinguished by two areas of area pellucida being responsible for formation of embryonic tissues and primordial germ cells, and area opaca forming the extra-embryonic tissues. Primordial germ cells are multi-potent stem cells giving rise to spermatogonia or oogonia. The present study was carried out to describe the characteristics of primordial germ cells in stage X of pheasants’ embryo using a transmission electron microscope. The blastoderm was dissected out from embryos which were already incubated for 12 hr. Toluidine blue was used for staining semi-thin sections; lead citrate and uranyl acetate were also used to stain ultra-thin sections. Images of primordial germ cells elucidated that the nucleus was situated eccentrically and had a compact spherical structure. Moreover, the nucleolus appeared elongated and was located eccentrically. The cytoplasm was composed of yolk granules and glycogen particles. Mitochondria were observed as round structures in the cytoplasm. The most important finding was that the primordial germ cells contained yolk granules, mitochondria and small amount of glycogen at this stage.
Nasser Darban Maghami; Abolghasem Nabipour; Mohammad Mohsenzadeh; Maryam Torabi
Volume 13, Issue 1 , March 2022, , Pages 47-53
Abstract
Meat and meat products are highly important sources of protein in the diet. Nowadays, the consumption of meat and meat products has increased owing to modern manufacturing techniques. Due to the economic value of meat, the use of unauthorized tissue is possible in meat products. In some cases, there ...
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Meat and meat products are highly important sources of protein in the diet. Nowadays, the consumption of meat and meat products has increased owing to modern manufacturing techniques. Due to the economic value of meat, the use of unauthorized tissue is possible in meat products. In some cases, there is fraud in the percentage of meat in meat products to reduce prices. In this study, 34 samples of minced meat, hamburger and sausage were randomly collected from the markets in the northeast of Iran. Then, sections were stained using Hematoxylin and Eosin (H & E), Verhoeff-van-Gieson, Masson's trichrome and periodic acid–Schiff-Alcian blue stains. In this regard, for the first time, the efficacy of stereological technique to determine the percentage of meat listed in sausages and the possible existence of fraud was evaluated. The results showed that, due to the presence of some unusual tissues, histological technique could determine different tissues in meat products. The stereological results of control samples showed a very slight difference; whereas, the results for the samples collected from the city stores showed a distinctive difference regarding the percentage of meat compared to the percentage of label. Skeletal and smooth muscles, blood vessels, nerve, gizzard, adipose tissue, glandular tissue, cartilage, bone, tendon, skin, lymphatic tissues and plant materials were observed. It was confirmed that stereology was a reliable method to determine and confirm the percentage of meat used in meat products.