Pathology
Emin Karakurt; Nuvit Coskun; Uğur Aydın; Serpil Dağ; Enver Beytut; Veysel Soydal Ataseven; Volkan Yılmaz; Fırat Doğan; Hilmi Nuhoğlu; Celal Şahin Ermutlu; Ayfer Yıldız
Volume 15, Issue 2 , February 2024, , Pages 75-82
Abstract
This study was aimed at the evaluation of cell proliferation, p53 level and apoptotic index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, ...
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This study was aimed at the evaluation of cell proliferation, p53 level and apoptotic index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Cases of canine oral papillomatosis, determined to have been caused by canine papillomavirus-1, were found to have a rather high cell proliferation index. Furthermore, all cases were immunohisto-chemically demonstrated to carry a mutant p53 gene. Despite the mutation of p53 gene, the shift in the Bax/Bcl-2 ratio of dogs diagnosed with tumor was in favor of the pro-apoptotic Bax gene. The apoptotic mechanism was determined to occur through both the caspase-dependent and caspase-independent pathways. While the lesions occupied the entire oral cavity in some cases, histopathologically, malignant transformation was not detected in any of the six cases.
Takuma Aoki; Seiya Niimi
Volume 14, Issue 4 , April 2023, , Pages 237-241
Abstract
Acute pulmonary edema in puppies generally occurs due to congenital left-right shunts such as patent ductus arteriosus or large ventricular septal defects. Herein, we presented two cases of puppies with no apparent congenital cardiovascular disease. Case 1: A 12-day-old male Labrador Retriever, weighing ...
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Acute pulmonary edema in puppies generally occurs due to congenital left-right shunts such as patent ductus arteriosus or large ventricular septal defects. Herein, we presented two cases of puppies with no apparent congenital cardiovascular disease. Case 1: A 12-day-old male Labrador Retriever, weighing 1.15 kg, was unable to suckle sufficiently from its dam and exhibited laboured breathing. Pulmonary edema was identified in all lung lobes by radiography, furthermore, echocardiography revealed significant enlargement of the left side of the heart. Pulmonary edema secondary to volume overload was suspected and furosemide was administered. The respiratory status was improved on the following day. Pimobendan was administered orally in addition to furosemide and both were withdrawn 6 weeks later when the heart size was normalized. Case 2: A 15-day-old female Standard Poodle, weighing 0.68 kg, was less active than other littermates and exhibited laboured breathing. Radiography revealed pulmonary edema in the right posterior lobe, dilatation of the caudal vena cava and ascites. Echocardiography revealed significant enlargement of the left atrium and ventricle perhaps owing to decreased left ventricular contractility. Furosemide and pimobendan were administered. One week later, appetite was improved and supraventricular tachycardia of 375 bpm was observed. Therefore, tachycardia-induced dilated cardiomyopathy was suspected which returned to sinus rhythm with diltiazem treatment, however, it was recurred. Upon sotalol monotherapy, a normal heart size was observed seven months later. In conclusion, we encountered two new-born puppies with transient pulmonary edema that were temporarily treated with pimobendan and furosemide.
Sibel Hasircioğlu
Volume 14, Issue 3 , March 2023, , Pages 113-119
Abstract
Canine parvovirus type 2 (CPV-2) causes hemorrhagic enteritis, and is one of the most important and contagious pathogens of dogs. In this study, we aimed to determine the prevalence and antigenic variants of CPV enteritis in dogs. Fecal samples were collected from 35 dogs with mucoid to hemorrhagic diarrhea ...
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Canine parvovirus type 2 (CPV-2) causes hemorrhagic enteritis, and is one of the most important and contagious pathogens of dogs. In this study, we aimed to determine the prevalence and antigenic variants of CPV enteritis in dogs. Fecal samples were collected from 35 dogs with mucoid to hemorrhagic diarrhea in the Western Mediterranean region of Türkiye between October 2019 and March 2021. DNA was isolated from the samples and examined using PCR analysis. Twenty-eight out of 35 dogs (80.00%) were detected to be positive for CPV. Of these, three had already been vaccinated. The partial VP2 genes of 15 CPV positive samples producing strong bands in agarose gels were sequenced. All strains were identified as CPV-2b, and the amino acid changes were identified. Discriminative amino acid changes were detected for different amino acid positions clearly defining new CPV-2b variants. Of the 15 isolates, three had previously unreported synonymous mutations. Phylogenetic analysis indicated that the strains obtained in this study were closely related to isolates from the Mersin province of Türkiye, except for three isolates that had synonymous mutations and were located in a separate branch from the other CPV-2b genetic variants previously detected in Mersin Province and Urfa Province in Türkiye. This study demonstrates the increase in the prevalence rates for CPV-2b circulating in vaccinated and nonvaccinated dogs. Taking into account the data from phylogenetic trees which highlights differences between the vaccine strains and the isolates, re-designing immunization strategies needs necessary.
Ibrahim Dorrigiv; Mojtaba Hadian; Morteza Bahram
Volume 14, Issue 3 , March 2023, , Pages 169-176
Abstract
Volatile compounds of anal sac secretions are odorant chemicals used across the carnivores for social communication such as identifying individuals and group membership. Odor profiles taken from expressed anal sac secretions of some species of carnivores have been detected in previous studies. In this ...
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Volatile compounds of anal sac secretions are odorant chemicals used across the carnivores for social communication such as identifying individuals and group membership. Odor profiles taken from expressed anal sac secretions of some species of carnivores have been detected in previous studies. In this study, the volatile compounds of anal sac secretions between five male and five female domestic dogs (Canis lupus familiaris) were compared. Volatile chemicals were extracted, separated, and analyzed by gas chromatography-mass spectrometry with solid-phase micro-extraction and identified from their electron ionization mass spectra and Kovats retention indices. The results showed the presence of various types of compounds including organic fatty acids, ketones, aldehydes, esters, and alcohols in the anal sac secretions of dogs. Greater amounts of diversity and esters, and lower amounts of alcohols were detected in the anal sac secretions of females compared to males. This was accompanied by finding citrate and acetic acid ester only in the females. Furthermore, presence of some sex-specific organic compounds like dimethylcyclopentyl ethanone indicates that the volatile profiles of anal sac secretions in 10 domestic dogs are differentiated by host sex.
Mohammad Sadegh Ashtari; Abbas Veshkini; Majid Masoudifard; Hassan Gilanpour; Alireza Bahonar
Volume 12, Issue 4 , December 2021, , Pages 493-496
Abstract
Evaluation of pelvic diaphragm muscles in dogs merits clinical attention because of the anatomical importance and their involvement in perineal hernia. This study aimed to evaluate the normal pelvic diaphragm muscles (levator ani and coccygeus muscles) using the computed tomography (CT) scan. For this ...
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Evaluation of pelvic diaphragm muscles in dogs merits clinical attention because of the anatomical importance and their involvement in perineal hernia. This study aimed to evaluate the normal pelvic diaphragm muscles (levator ani and coccygeus muscles) using the computed tomography (CT) scan. For this purpose, 10 male and 10 female clinically normal, adult intact mixed-breed dogs weighing 15.00 to 20.00 kg were selected randomly. All animals underwent CT examination under general anesthesia and positioned in sternal recumbency. All the CT images were taken and reconstructed with a same protocol. The measurements of the pelvic diaphragm muscles were performed based on the presented guideline to the observer. The results were presented descriptively. The mean lengths of the coccygeus muscle in CT images were evaluated 4.60 cm for males and 4.30 cm for females. The mean lengths of levator ani muscle in CT images were measured 4.90 cm in males and 4.50 cm in females. In conclusion, the results of the present study can be beneficial to clinicians and researchers for further evaluation of the supporting muscles in pelvic cavity and underlying anatomical changes in perineal hernia in dogs.
