Microbiology
Heidar Rahimi; Amir Tukmechi; Ehsan Rashidian
Articles in Press, Corrected Proof, Available Online from 08 August 2023
Abstract
The genetic diversity of Brucella strains has not been fully understood. To investigate this, the genetic characteristics of 64 isolates of Brucella melitensis from sheep and goats’ milk were studied using random fragment length polymorphism (RFLP) and multiple locus variable-number tandem repeat ...
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The genetic diversity of Brucella strains has not been fully understood. To investigate this, the genetic characteristics of 64 isolates of Brucella melitensis from sheep and goats’ milk were studied using random fragment length polymorphism (RFLP) and multiple locus variable-number tandem repeat analysis (MLVA-16) methods developed in Orsay, France (MLVA-16Orsay). The RFLP analysis revealed that all 64 isolates were of biovar one. The MLVA-typing showed that one sample was simultaneously infected with two strains of B. melitensis and the genotype of 65 isolate was analyzed. Four genotypes (47, 42, 43, and 63) were identified using MLVA-8 (panel 1), whereas six genotypes (138, 125, 116, 108, and two unknown genotypes) were identified using MLVA11 (panels 1 and 2A). From the review of MLVA-16 (panels 1, 2A, and 2B), panel 2B showed a very high discriminatory power. Two loci of Bruc04 and Bruc30 from this panel had diversity index values higher than 0.71 and the average diversity index was 0.619. So MLVA-16Orsay 34 showed the genotype indicating a low genetic homogeneity among the isolates. The findings of MLVA genotyping of the isolates suggest that strains of B. melitensis isolated from the milk of small ruminants in Iran are most closely related to the isolates from neighboring countries of the Eastern Mediterranean group. To the best of our knowledge, this is the first study to indicate the potential use of MLVA genotyping for simultaneous detection of specimen contamination using two different B. melitensis biovars.
Microbiology
Sushila Maan; Anita Dalal; Naresh Kumar Kakker; Deepika Chaudhary; Aman Kumar; Narender Singh Maan
Articles in Press, Accepted Manuscript, Available Online from 24 October 2023
Abstract
Abstract: World Organization for Animal Health has listed bluetongue (BT) under notifiable diseases. BT is an arboviral infectious disease of domestic and wild ruminants caused by the bluetongue virus (BTV). Southern states of India had remained the point of attention for BT since first presence in 1964 ...
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Abstract: World Organization for Animal Health has listed bluetongue (BT) under notifiable diseases. BT is an arboviral infectious disease of domestic and wild ruminants caused by the bluetongue virus (BTV). Southern states of India had remained the point of attention for BT since first presence in 1964 in Maharashtra. Recently, northern states of India have also been reported positive for BTV in small ruminants. The present study reports the dual infection of BTV serotypes, BTV-12 and -16 in sheep population from Sirsa district of Haryana in the year 2016. After detection and serotyping with Seg-2 specific real time PCR, the Seg-2 and Seg-6 of BTV were PCR amplified and sequenced. On phylogenetic analysis it was detected to be clustered in Nucleotype G and Nucleotype B specific for BTV-12 and BTV-16, respectively. This is the first report of BTV-16 from Haryana. The results signify the co-infection of two different serotypes in an animal from a single outbreak.
Parasitology
Khadijeh Seydi Gazafi; Mousa Tavassoli; Karim Mardani
Volume 14, Issue 9 , September 2023, , Pages 489-494
Abstract
Pyrethroid insecticides target voltage-gated sodium channels (VGSCs) that are essential for electrical signaling in the nervous system of insects. Three-point mutations at the corresponding amino acid sequence positions M815I, T917I, and L920F located in domain II conferring the knockdown resistance ...
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Pyrethroid insecticides target voltage-gated sodium channels (VGSCs) that are essential for electrical signaling in the nervous system of insects. Three-point mutations at the corresponding amino acid sequence positions M815I, T917I, and L920F located in domain II conferring the knockdown resistance (kdr) are the most important mutations in pyrethroid-resistant lice worldwide. In addition, six new mutations have been reported in the extracellular loops IIS1-2 (H813P) and IIS5 (I927F, L928A, R929V, L930M, L932M) in the α- subunit of the sodium channel in lice. The aim of this study was to detect alleles resistant to pyrethroids in the domain II (S5-S6) of the VGSC gene in goat biting louse. Goat biting lice were collected from five provinces in the west and northwest of Iran. Genomic DNA was extracted from goat biting lice and Bovicola (Damalinia) caprae species was confirmed by amplifying the cytochrome oxidase subunit I (COXI) gene. A fragment in the domain II (S5-S6) of the VGSC gene was amplified using the specific primers and the resultant polymerase chain reaction products were sequenced. Substitutions T917I, L920F, I927F, L928A, R929V and L930M were identified in the examined sequences. The results showed that all the examined lice had at least one mutation in their VGSC gene associated with pyrethroid resistance or new mutations. The presence of these mutated alleles in the VGSC gene may be due to the long-term and multiple use of pyrethroids against arthropods. Thus, the molecular detection of resistance to pyrethroid insecticides in goat chewing lice can help plot a kdr frequency map to enact effective policies to control caprine pediculosis.
Parasitology
Shivan Nawzad Hussein; Assel Anwer Ibrahim; Mohammed Shukri Shukur
Volume 14, Issue 8 , August 2023, , Pages 415-422
Abstract
This study aimed to identify Sarcocystis species isolated from macroscopic sarcocysts of naturally infected sheep and goats using histopathological and molecular studies. A total of 260 macrosarcocyst samples were randomly collected from 1,337 infected sheep and goats slaughtered at different abattoirs ...
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This study aimed to identify Sarcocystis species isolated from macroscopic sarcocysts of naturally infected sheep and goats using histopathological and molecular studies. A total of 260 macrosarcocyst samples were randomly collected from 1,337 infected sheep and goats slaughtered at different abattoirs in Duhok province, Iraq, from May 2021 to June 2022. The macroscopic cysts, which were found in the esophagus, diaphragm, and abdominal muscles, were classified into fat and thin cysts. Histopathological examination of the observed fat and thin cysts showed a thick eosinophilic wall, several internal septa-forming compartments enclosing numerous bradyzoites, and mild mononuclear inflammatory cells infiltrating around the cysts. The 18 Subunit ribosomal RNA (18S rRNA) and 28 Subunit ribosomal RNA (28S rRNA) genes of Sarcocystis spp. were amplified by polymerase chain reaction (PCR) from 200 macrosarcocysts samples. Molecularly, the DNA sequencing results obtained from fat macrocysts of sheep and goats were found to be identical to Sarcocystis gigantea, and from thin cysts of sheep proved to be similar to Sarcocystis medusiformis, while from thin macrocysts of goats were found to be identical to Sarcocystis moulei. Alignment and phylogenetic analysis observed a very close relationship between identified species of Sarcocystis and other Sarcocystis DNA sequences of sheep and goats across the world. To our knowledge, this is the first histopathological and molecular study for identification of Sarcocystis spp. isolated from different macroscopic forms of sarcocysts of sheep and goats in Iraq.
Mostafa Abdollahi; Samad Lotfollahzadeh; Mohammad Hossein Nazem Shirazi; Sara Shokrpoor; Farhad Moosakhani; Minoo Partovi Nasr
Volume 14, Issue 2 , February 2023, , Pages 109-112
Abstract
Mycoplasma capricolum subspecies capripneumoniae (Mccp) is the etiological agent of caprine contagious pleuropneumonia (CCPP) disease. The CCPP is one of the most severe diseases of goats. A herd of 2,000 goats located in the countryside of Tehran city, Iran, was examined for the study in August 2021. ...
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Mycoplasma capricolum subspecies capripneumoniae (Mccp) is the etiological agent of caprine contagious pleuropneumonia (CCPP) disease. The CCPP is one of the most severe diseases of goats. A herd of 2,000 goats located in the countryside of Tehran city, Iran, was examined for the study in August 2021. In history taking, observation, inspection and clinical examination, high case fatality rate (46.00%) due to respiratory distress and high morbidity of pleuropneumonia (15.00%) syndrome were recorded. Accordingly, ten carcasses of goats were dissected. The epidemiological pattern of the disease, clinical examination findings and the signs of necropsy of dead patients were suspected to CCPP. Four lung samples of necropsied goats were sent to the laboratory for PCR test and in all of them, Mccp was detected and CCPP was also confirmed. The disease was controlled by two measures: (a) the whole herd was first treated with antibiotics (florfenicol and tylosin) and (b) then the Pulmovac-In vaccine was then administered. This study is the first documented report of CCPP occurrence caused by Mccp in Iran and shows the importance of availability of effective vaccines to control and prevention of CCPP.
Elham Hassan; Ahmed Abdelgalil; Faisal Torad
Volume 11, Issue 1 , March 2020, , Pages 1-5
Abstract
Ultrasonography is a safe, rapid, and non-invasive diagnostic tool that has been previously used for imaging infants and canine neonatal brains. The purpose of the present study was to describe the ultrasonographic appearance of the brain in clinically normal caprine neonates. Ultrasonographic examination ...
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Ultrasonography is a safe, rapid, and non-invasive diagnostic tool that has been previously used for imaging infants and canine neonatal brains. The purpose of the present study was to describe the ultrasonographic appearance of the brain in clinically normal caprine neonates. Ultrasonographic examination was done on 12 day-old goat kids, transverse and sagittal transcranial scans were obtained through the frontal bone. Three image planes were recorded through transverse scans including plane I (level of the caudate nucleus), plane II (level of the rostral diencephalon) and plane III (level of the caudal diencephalon). Parallel post mortem examinations were done for two kids that died a day following examination due to accidental trauma by the dam. Reliable and repeatable ultrasonographic images of the goat kid’s brain were described based on the gross post mortem findings. The head of the caudate nucleus was taken as an anatomical landmark in the plane I where it appeared as a curved hyperechoic structure. In plane II, the longitudinal fissure with its characteristic umbrella-like structure was taken as a landmark, while in plane III, the laterally located hyperechoic hippocampus was taken as a landmark. Normal ultrasonographic examination of the caprine neonatal brain represented the basis for diagnosing congenital brain lesions as well as intracranial hemorrhage.
Large Animal Internal Medicine
Abdollah Derakhshandeh; Fatemeh Namazi; Elmira Khatamsaz; Vida Eraghi; Zahra Hemati
Volume 9, Issue 3 , September 2018, , Pages 253-257
Abstract
In the present study, Mycobacterium avium subsp. paratuberculosis (MAP) was investigated in goats slaughtered in Shiraz abattoir using histopathological examinations and polymerase chain reaction (PCR). Ilium and mesenteric lymph node samples from 66 suspected goat carcasses to Johne’s disease ...
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In the present study, Mycobacterium avium subsp. paratuberculosis (MAP) was investigated in goats slaughtered in Shiraz abattoir using histopathological examinations and polymerase chain reaction (PCR). Ilium and mesenteric lymph node samples from 66 suspected goat carcasses to Johne’s disease were collected. Among 66 examined slaughtered goats, nine (13.63%) goats were positive for MAP in both histopathological and PCR examinations. Eight goats were positive in PCR method while no lesion related to Johne’s disease was observed in their histopathological sections. All positive goats in histopathological examination were also positive in PCR. Based on the results of PCR, the detection rate of MAP in Shiraz abattoir was 25.80% (17 goats). According to the present findings, although both histopathological and PCR methods are appropriate for detecting Johne’s disease, PCR is more sensitive than histopathological examination.
Parasitology
Seyyed Mostafa Mohammadi; Bijan Esmaeilnejad; Ghader Jalilzadeh-Amin
Volume 8, Issue 2 , June 2017, , Pages 139-144
Abstract
This study was aimed to determine the infection rate and vectors of Theileria lestoquardi in goats from West Azerbaijan province, Iran. A total of 400 blood samples were collected from 40 randomly selected flocks in the study area from June to September, 2014. Out of 400 blood samples examined using ...
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This study was aimed to determine the infection rate and vectors of Theileria lestoquardi in goats from West Azerbaijan province, Iran. A total of 400 blood samples were collected from 40 randomly selected flocks in the study area from June to September, 2014. Out of 400 blood samples examined using microscopic examination, a number of 14 goats (3.50%) were positive for Theileria spp., whereas 25 goats (6.25%) yielded a specific T. lestoquardi SSU-rRNA fragment (235 bp). The prevalence of theileriosis in goats estimated by semi-nested PCR was significantly higher than the prevalence estimated by microscopic examination of the blood smears. The prevalence of Theileria infection in different age and sex groups of goats was not significantly different. The highest and lowest prevalence of Theileria infection was in July (12.00%) and September (2.00%), respectively. A number of 315 adult Ixodid ticks were also collected from naturally infested goats and they were characterized. Out of 315 examined ticks, a number of 37 ticks including Hyalomma marginatum (65.20%), Rhipicephalus turanicus (44.00%), and Dermacentor marginatus (68.70%) were infected by T. lestoquardi. Based on the obtained results, it was concluded that the semi-nested PCR assay based on SSU-rRNA gene is a valuable method for epidemiological investigation of caprine theileriosis. The results showed that H. marginatum, R. turanicus and D. marginatus can be considered as risk factor in the epidemiology of T. lestoquardi.
Mohammad Arjmand; Abdolah Mirzaei; Mohammad Reza Jafarzadeh Shirazi; Amin Tamadon; Mohammad Saied Salehi; Mehdi Saeb; Mohammad Reza Namavar; Mohammad Reza Zandi; Hojatollah Shahheidari; Sara Moradi
Volume 5, Issue 4 , December 2014, , Pages 247-254
Abstract
The aims of the present study were to evaluate luteal activity in Abadeh ecotype goat during summer and winter and also the effect of a single dose kisspeptin-10 injection on the secretion of luteinizing hormone (LH) in female anestrous goats. In the first study, progesterone (P4) concentration in 10 ...
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The aims of the present study were to evaluate luteal activity in Abadeh ecotype goat during summer and winter and also the effect of a single dose kisspeptin-10 injection on the secretion of luteinizing hormone (LH) in female anestrous goats. In the first study, progesterone (P4) concentration in 10 goats in summer (n = 6) and winter (n = 4) were measured every other day. Moreover, in summer group, a male teaser goat was left in the herd on days of sampling for one hour. Goats with P4 concentration ≥1 ng mL-1, at least two consecutive measurements, were considered with luteal activity. In the second study, the anestrous phase was confirmed by P4 measurement 20 and 10 days before the kisspeptin injection in five female Abadeh ecotype goats (4 to 5 years old). The goats were given a single IV injection of saline (2 mL) as control group and the same goats (1 hr after the last blood sampling) were given kisspeptin (1 μg kg-1) as treatment group. The blood samples were collected at –60, –40, –20 and 0 min (before injection), and 10, 20, 30, 40, 50, 60, 80, 100, 120 and 140 min after the injection and LH concentration were measured. A single IV injection of 1 µg kg-1 of kisspeptin-10 did not stimulate the release of LH in female anestrous goats. In summer, in the presence of teaser goat, luteal activity was seen in all goats. In the absence of male goat in winter, some goats showed luteal activity and others showed anestrus.
Mahdi Deljou; Mohammad Reza Aslani; Mehrdad Mohri; Ahmad Reza Movassaghi; Mohammad Heidarpour
Volume 5, Issue 3 , July 2014, , Pages 161-167
Abstract
Toxic effects of monensin, a polyether antibiotic mainly used as a coccidiostat, have been described in a wide range of animals. The present study was performed to investigate the toxic effects of monensin in goats. Seven adult goats were administered sodium monensin, 13.5 mg kg-1, daily for five consecutive ...
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Toxic effects of monensin, a polyether antibiotic mainly used as a coccidiostat, have been described in a wide range of animals. The present study was performed to investigate the toxic effects of monensin in goats. Seven adult goats were administered sodium monensin, 13.5 mg kg-1, daily for five consecutive days via gastric gavage. Monensin toxicity was evaluated by clinical signs, serum biochemistry and pathology. Monensin exposure caused diarrhea, tachycardia and reduction in ruminal movements and body temperature. Significant increase of creatine kinase, lactate dehydrogenase, aspartate aminotransferase, total bilirubin, blood urea nitrogen, creatinine and erythrocyte superoxide dismutase were observed in monensin exposed goats. Reduction of erythrocyte glutathione peroxidase and elevation of serum malondialdehyde and troponin I were inconsistent. In necropsy, there were effusions in body cavities, vacuolar degeneration and coagulative necrosis in cardiac and skeletal muscles and renal tubular necrosis. These findings suggested that monensin intoxication in goats leads to cardiac, skeletal and renal damage and a wide range of biochemical abnormalities. Oxidative stress may be involved in the pathogenesis of monensin poisoning.
Mousa Tavassoli; Mohammad Ghorbanzadehghan; Bijan Esmaeilnejad
Volume 4, Issue 1 , March 2013, , Pages 43-47
Abstract
Infection by the protozoan parasite Toxoplasma gondii, is widespread in humans and many other warm-blooded animals. More than half billion of world human population has serum antibodies to T. gondii and Sheep and goats are more widely infected with T. gondii. T. gondii infection can be diagnosed indirectly ...
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Infection by the protozoan parasite Toxoplasma gondii, is widespread in humans and many other warm-blooded animals. More than half billion of world human population has serum antibodies to T. gondii and Sheep and goats are more widely infected with T. gondii. T. gondii infection can be diagnosed indirectly with serological methods and directly by polymerase chain reaction (PCR), hybridization, isolation and histology. A total number of 124 goats and 113 sheep blood samples were collected from Urmia region and PCR was used for detection of the pathogenic protozoan T. gondii using B1 gene. The targeted B1 gene is highly conserved in all T. gondii strains and is multiple copy genes whit in the T. gondii genome. The method used for the characterization of T. gondii strains implied digestion with AluI restriction enzyme of the fragments amplified. The results indicated three positive sheep (1.26%) with one RFLP patterns. The results indicated that the same strain of T. gondii has infected sheep in the region.
Yacob Garedaghi
Volume 2, Issue 2 , June 2011, , Pages 129-133
Abstract
Linguatula serrata is one of well-known members of Pentastomida which infects both humans and animals. The aim of this study was to evaluate the prevalence of L. serrata in mesenteric lymph nodes, livers and lungs of goats slaughtered in Tabriz area, Iran. Mesenteric lymph nodes (MLNs), livers and lungs ...
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Linguatula serrata is one of well-known members of Pentastomida which infects both humans and animals. The aim of this study was to evaluate the prevalence of L. serrata in mesenteric lymph nodes, livers and lungs of goats slaughtered in Tabriz area, Iran. Mesenteric lymph nodes (MLNs), livers and lungs of 280 goats slaughtered in Tabriz, North-west of Iran were investigated for nymphs of L. serrata from September 2009 to September 2010. The organs were examined macroscopically and then a tissue digestion method was also done for investigation of liver and lung of the goats that were infected MLN. The liver and lung samples were mostly taken from the condemned and rejected part of organs. The infection rate of L. serrata nymphs in MLNs, livers and lungs was 27.1 %, 2.8 % and 2.8 % respectively. The number of isolated nymph in infected lymph nodes varied from 1 to 22 with a mean of 7. Only one nymph was isolated from each infected livers and lungs. The infection rate increased with age (P < 0.05). No significant difference in different sex groups and seasons was observed (P > 0.05). Linguatula infection occurs as an endemic zoonosis in the study area and has an active transmission life cycle.
