Pathology
Ömer Faruk Keleş
Articles in Press, Accepted Manuscript, Available Online from 13 March 2024
Abstract
Linguatulosis can infect most ruminants and cause accidental infections in humans. Linguatula serrata is the causative parasite of Linguatulosis, a disease that not only produces economic losses in cattle but also represents a public health risk due to its zoonotic nature. This study aimed to explore ...
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Linguatulosis can infect most ruminants and cause accidental infections in humans. Linguatula serrata is the causative parasite of Linguatulosis, a disease that not only produces economic losses in cattle but also represents a public health risk due to its zoonotic nature. This study aimed to explore the clinical and pathologic findings of pulmonary linguatulosis in a rabbit. The most striking clinical findings in the deceased rabbits were wheezing and labored breathing. Grossly, the most prominent morphological changes in the lungs were well-circumscribed, flat or slightly raised, solitary grayish-white nodular lesions and consolidated areas. The developmental forms of parasite with characteristically tongue-shaped were observed on cut surface of the lung. Histopathologically, the most striking morphological changes in the lung parenchyma were diffuse thickening of the interalveolar septum, fibrinoid necrotic vasculitis, hypertrophy of the medial smooth muscle cells of the arteries, alveolar emphysema, longitudinal and transverse sections of L. serrata nymphs and extramedullary hematopoietic foci (megakaryocytes). Morphologic appearance of the nymphs showed multiple transverse grooves, saw-like cuticles, peribuccal hooks and acidophilic glands. In conclusion, these findings reveal the etiopathological diagnosis of Linguatulosis and suggest that the lungs might be a target organ in addition to the liver and lymph nodes.
Pathology
Tomokazu Nagashima; Chisato Kishi; Yukino Machida; Masaki Michishita
Articles in Press, Accepted Manuscript, Available Online from 13 March 2024
Abstract
A 16-year-old male mixed-breed dog presented with a mass with hemorrhage at the right conjunctiva. Five months after the initial visit, the right eye protruded and had a firm and irregular mass measuring approximately 1 cm in diameter with conjunctival hemorrhage. Microscopically, the mass was comprised ...
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A 16-year-old male mixed-breed dog presented with a mass with hemorrhage at the right conjunctiva. Five months after the initial visit, the right eye protruded and had a firm and irregular mass measuring approximately 1 cm in diameter with conjunctival hemorrhage. Microscopically, the mass was comprised polygonal or round tumor cells with distinct cell borders arranged in a nested and diffuse pattern. The tumor cells had round-to-oval fine hyperchromatic nuclei containing distinct multiple nucleoli and abundant eosinophilic or pale cytoplasm. Multiple giant cells were frequently observed. The mitotic index was 12.6/high power field. Extensive necrosis, hemorrhage and part of the cord-like or papillary epithelioid cells were observed in the intratumor tissue. Immunohistochemically, the tumor cells were positive for vimentin and α-smooth muscle actin and negative for cytokeratin, desmin and PNL2. On the other hand, the cord-like and papillary epithelioid cells were positive for vimentin, S100 and NSE. The tumor was diagnosed as an epithelioid leiomyosarcoma. This case considered to have occurred in the ocular region, although the ocular structure was destroyed.
Pathology
Osman Dağar; Mehmet Burak Ateş; Mustafa Ortatatlı; Fatih Mehmet Öztürk
Volume 15, Issue 4 , April 2024, , Pages 203-205
Abstract
This study presents the first report of congenital tuberculosis in an 8-month-old male British Shorthair cat. The case was examined using histopathological and immunohistochemical methods. The cat was referred to a private veterinary clinic with general respiratory system problems and subsequent deterioration, ...
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This study presents the first report of congenital tuberculosis in an 8-month-old male British Shorthair cat. The case was examined using histopathological and immunohistochemical methods. The cat was referred to a private veterinary clinic with general respiratory system problems and subsequent deterioration, leading to death. The cat owner granted permission for the cat necropsy and pathological examinations at Department of Pathology, Faculty of Veterinary Medicine, Selçuk University, Konya, Türkiye. During systemic necropsy, white round foci with diameters ranging from 3.00 to 5.00 mm were observed in the lung and spleen. Tissue samples were collected from the lung, spleen, liver, heart, kidney, mediastinal lymph nodes and brain for histopathological examinations. The tissues were subjected to routine histological tissue processing and sections were stained with Hematoxylin and Eosin and Ziehl-Neelsen. Additionally, Mycobacterium spp. antibodies were used for immunohistochemical staining. Microscopic examination revealed exudative tuberculosis lesions, areas of necrosis without a fibrous capsule and karyorrhectic cells only in the lung and spleen. Acid-resistant bacteria observed by ZN staining in the lesioned areas of the lung and spleen were identified as Mycobacterium spp. using immunohistochemical staining. No positive staining was observed in other organs using ZN and immunohistochemical methods. As a result, congenital tuberculosis was diagnosed in a cat for the first time, especially in relation to lesions in the spleen.
Pathology
Emin Karakurt; Nuvit Coskun; Uğur Aydın; Serpil Dağ; Enver Beytut; Veysel Soydal Ataseven; Volkan Yılmaz; Fırat Doğan; Hilmi Nuhoğlu; Celal Şahin Ermutlu; Ayfer Yıldız
Volume 15, Issue 2 , February 2024, , Pages 75-82
Abstract
This study was aimed at the evaluation of cell proliferation, p53 level and apoptotic index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, ...
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This study was aimed at the evaluation of cell proliferation, p53 level and apoptotic index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Cases of canine oral papillomatosis, determined to have been caused by canine papillomavirus-1, were found to have a rather high cell proliferation index. Furthermore, all cases were immunohisto-chemically demonstrated to carry a mutant p53 gene. Despite the mutation of p53 gene, the shift in the Bax/Bcl-2 ratio of dogs diagnosed with tumor was in favor of the pro-apoptotic Bax gene. The apoptotic mechanism was determined to occur through both the caspase-dependent and caspase-independent pathways. While the lesions occupied the entire oral cavity in some cases, histopathologically, malignant transformation was not detected in any of the six cases.
Pathology
Amir Ali Shahbazfar; Marzieh Heidarieh; Samira Shahbazi; Hamed Askari
Volume 14, Issue 11 , November 2023, , Pages 607-614
Abstract
Trichoderma species are considered as biological control agents against numerous phytopathogenic fungi. They are also helpful for plants as plant symbiont. This study aimed to identify harmful effects of Trichoderma in laboratory animals. In the first step, inhalation toxicity was studied. Six rats as ...
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Trichoderma species are considered as biological control agents against numerous phytopathogenic fungi. They are also helpful for plants as plant symbiont. This study aimed to identify harmful effects of Trichoderma in laboratory animals. In the first step, inhalation toxicity was studied. Six rats as control received a spray of bio-formulation without spores. Ten rats as treatment A received 1.00 × 106 colony-forming unit (CFU) of Trichoderma spores and ten rats as treatment B received 1.00 × 107 CFU per test of Trichoderma spores. The harmful effects of Trichoderma were obvious especially in the lungs, liver and kidney, and some blood parameters were abnormal. In the second step, we studied acute oral toxicity by gavage. Four rats as control received bio-formulation without spores. Six rats as treatment A received 1.00 × 106 CFU per test of Trichoderma spores. Six rats as treatment B received 1.00 × 107 CFU per test of Trichoderma spores. The harmful effects of Trichoderma were noticeable more in the liver and kidney tissues. For dermal toxicity study, two rabbits as control received bio-formulation without spores by rubbing on the surface of the skin. Treatment groups A and B received 1.00 × 106 and 1.00 × 107 CFU per test of Trichoderma spores, respectively (four rabbits for each group). The liver and kidney and some blood parameters were abnormal. Trichoderma has some harmful effects on tissues and organs and although it is a natural product, it should be used under cautions.
Pathology
Ayhan Atasever; Ali Sefa Mendil; Mehmet Ozkan Timurkan
Volume 14, Issue 10 , October 2023, , Pages 541-548
Abstract
This study aimed to investigate the potential presence of bovine herpes virus type 1 (BHV-1) and bovine viral diarrhea virus (BVDV) in cattle uteri that did not display any clinical and macroscopic signs of infection. Virus detection involved polymerase chain reaction (PCR) test, double immunohistochemistry ...
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This study aimed to investigate the potential presence of bovine herpes virus type 1 (BHV-1) and bovine viral diarrhea virus (BVDV) in cattle uteri that did not display any clinical and macroscopic signs of infection. Virus detection involved polymerase chain reaction (PCR) test, double immunohistochemistry (IHC), and double immunofluorescence (IF). One hundred cornu uterus samples were collected from cattle aged 1 year and older. The BVDV was detected by PCR or by double IHC/IF in the collected samples from slaughterhouses in Kayseri city (Central Anatolia, Türkiye) from 2021 - 2022. By contrast, BHV-1 was detected by PCR and double IHC/IF at a rate of 16.00% and 21.00%, respectively. In the IHC and IF detection, BHV-1 was detected in endometrial epithelial cells and in some mononuclear cells in the lamina propria, periglandular areas and myometrium. Although no macroscopic lesion was found in the BHV-1-positive samples (n = 21), histopathological detection showed that two had acute endometritis, eight had subacute endometritis, eight had chronic endometritis and the three others showed no signs of endometritis. This prevalence study demonstrated for the first time that even while BVDV could not be detected in the samples, BHV-1 posed a critical potential reproductive risk in pregnant animals, as it can specifically cause abortions when it resides in cattle uteri that do not show clinical or macroscopic and even microscopic signs of infection. Additionally, this study was the first to combine PCR and double IHC/IF for BHV-1 and BVDV detection in cattle uteri.
Pathology
Hossein Alavizadeh; Zahra Moosavi
Volume 14, Issue 6 , June 2023, , Pages 347-350
Abstract
A 15-year-old male terrier dog with symptoms of lethargy and severe abdominal distension was referred to the polyclinic hospital of the Ferdowsi University of Mashhad, Mashhad, Iran. In addition to numbness and abdominal distension, the dog also had anorexia and severe weakness and some skin masses were ...
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A 15-year-old male terrier dog with symptoms of lethargy and severe abdominal distension was referred to the polyclinic hospital of the Ferdowsi University of Mashhad, Mashhad, Iran. In addition to numbness and abdominal distension, the dog also had anorexia and severe weakness and some skin masses were observed. Due to the enlarged abdomen, splenomegaly was diagnosed in ultrasonography. Fine needle aspiration was performed on the liver and skin mass and then, neoplastic lesions were reported based on cytology. On the necropsy, two masses were found on the liver and shoulder skin. These masses were well-encapsulated, soft and multi-lobulated. Samples taken from the liver and skin were prepared by Hematoxylin and Eosin staining and then, two different immunohistochemical markers were used to confirm the initial diagnosis. Histopathological examination of these two well-encapsulated, soft and multi-lobulated masses on the liver and skin showed lipid content and liposarcoma was indicated. Immunohistochemical staining using two markers, S100 and MDM2, made a definitive diagnosis and confirmed the diagnosis.
Pathology
Sadat Ghafarzadeh; Rahim Hobbenaghi; Esmaeal Tamaddonfard; Amir Abbas Farshid; Mehdi Imani
Volume 10, Issue 4 , December 2019, , Pages 277-284
Abstract
Crocin is a plant-derived carotenoid and bears potent antioxidant property. Ranitidine (a histamine H2 receptor blocker) is used for peptic ulcer treatment. The present study was planned to investigate the effects of crocin and ranitidine on indomethacin-induced ulcer in small intestine of rats. Animals ...
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Crocin is a plant-derived carotenoid and bears potent antioxidant property. Ranitidine (a histamine H2 receptor blocker) is used for peptic ulcer treatment. The present study was planned to investigate the effects of crocin and ranitidine on indomethacin-induced ulcer in small intestine of rats. Animals were randomized into two major groups including indo-methacin (10.00 mg kg-1, ulcer group, 48 rats) and normal saline (1.00 mL kg-1, intact group, 48 rats) groups. Each of these two major groups was subdivided into eight subgroups for intra-peritoneal (IP) injections of normal saline, crocin (2.50, 10.00 and 40.00 mg kg-1), ranitidine (5.00 and 20.00 mg kg-1), crocin (2.50 and 10.00 mg kg-1) plus ranitidine (5.00 mg kg-1). Indomethacin induced intestinal ulcer was characterized by bleeding, inflammation, epithelial hyperplasia and crypt loss. This non-steroidal anti-inflammatory drug (NSAID), indomethacin decreased goblet cell number and superoxide dismutase (SOD) activity and increased small intestine weight, organo-somatic index (OSI), malodealdehyde (MDA), tumor necrosis factor-α (TNF-α) and caspase-3 contents of intestine. Crocin resolved all the above-mentioned parameter changes induced by indomethacin. These treatments produced no significant effects on the above-mentioned parameters of intact group. The results of the present study showed tissue protective and anti-ulcer effects of crocin on small intestine by antioxidant, anti-inflammatory and anti-apoptotic mechanisms. Ranitidine alone showed no effect; however, in combination with crocin it exerted recovery effects. It is recommended that crocin, be considered as a therapeutic agent for NSAIDs-induced intestinal damage management.
Pathology
Amir Faramarzpour; Ali Asghar Tehrani; Esmaeal Tamaddonfard; Mehdi Imani
Volume 10, Issue 3 , September 2019, , Pages 227-234
Abstract
Crocin, as a carotenoid compound of saffron, exerts a potent antioxidant property. Mesalazine is frequently used in the treatment of ulcerative colitis. This study investigated the effects of separated and combination treatments with crocin and mesalazine in a rat model of ulcerative colitis. Ulcerative ...
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Crocin, as a carotenoid compound of saffron, exerts a potent antioxidant property. Mesalazine is frequently used in the treatment of ulcerative colitis. This study investigated the effects of separated and combination treatments with crocin and mesalazine in a rat model of ulcerative colitis. Ulcerative colitis was induced by intra-colonic administration of acetic acid (4.00%, 1.00 mL) at 8 cm proximal of the anus. Normal saline, acetic acid, crocin (5.00, 10.00 and 20.00 mg kg-1), mesalazine (100 and 300 mg kg-1) and crocin (5.00 mg kg-1) plus mesalazine (100 mg kg-1) were administered after induction of colitis for eight days. Body weight, oraganosomatic index (OSI), macroscopic and microscopic evaluations of colon and measurement of malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor-alpha (TNF-α) contents of colon tissue were determined on day eight after induction of colitis. Crocin (10.00 and 20.00 mg kg-1), mesalazine (300 mg kg-1) and crocin (5.00 mg kg-1) plus mesalazine (100 mg kg-1) significantly (p < 0.05) improved body weight and OSI and reduced macroscopic and microscopic scores. These treatments also significantly (p <0.05) recovered the increased levels of MDA and TNF-α as well as the decreased level of SOD in colon tissue. Crocin and mesalazine did not produce significant effects in intact rats. Based on the results, it is concluded that crocin and mesalazine produced protective effects on colon tissue via antioxidant and anti-inflammatory actions. In addition, a synergistic effect was observed between crocin and mesalazine in attenuating ulcerative colitis.
Pathology
Enrico Pierluigi Spugnini; Andrea Lanza; Silvia Sebasti; Alfonso Baldi
Volume 9, Issue 4 , December 2018, , Pages 379-381
Abstract
A five-year-old female African hedgehog (Atelerix albiventris) was referred for a one month growing oral mass. The hedgehog was quiet, alert and responsive, with a 1.00 × 1.50 cm ulcerated lesion on the mandible. The patient was staged with total body radiographs, hematological and biochemical ...
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A five-year-old female African hedgehog (Atelerix albiventris) was referred for a one month growing oral mass. The hedgehog was quiet, alert and responsive, with a 1.00 × 1.50 cm ulcerated lesion on the mandible. The patient was staged with total body radiographs, hematological and biochemical analyses and the mass was biopsied under anesthesia. The excised tissue was reported to be a squamous cell carcinoma. At this time, due to the extension of the mass, a surgical excision was not a therapeutic option and the tumor was palliated with electrochemotherapy (ECT). Monthly sessions of ECT were performed using intra-lesional bleomycin injection followed by trains of biphasic electric pulses. The treatment was well tolerated and the patient remained in partial remission for five months until tumor progression. At that time the pet was euthanized. The ECT resulted in improved local control and should be considered as a possible adjuvant treatment in exotic pets with advanced tumors.
Small Animal Internal Medicine
Hamed Mansour Lakooraj; Mahmood Ahmadi-hamedani; Omid Dezfoulian; Masoud Selk Ghaffari
Volume 9, Issue 3 , September 2018, , Pages 285-288
Abstract
A 10-year-old, male Rottweilerdog was presented to Small Animal Hospital of Tehran University with a history of lethargy, anorexia, weight loss, vomiting, polyuria, polydipsia and blindness. The dog showed symptoms of depression, high body temperature (39.2 ˚C), tachypnea (40 breaths min-1) and cachexia. ...
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A 10-year-old, male Rottweilerdog was presented to Small Animal Hospital of Tehran University with a history of lethargy, anorexia, weight loss, vomiting, polyuria, polydipsia and blindness. The dog showed symptoms of depression, high body temperature (39.2 ˚C), tachypnea (40 breaths min-1) and cachexia. In ophthalmic examination, bilateral hyphema (hemmorrahge in anterior chamber of the eye) and blindness were detected. The pulse of the animal was normal (90 beats min-1) and obvious general lymphadenopathy was determined. Lymph nodes were firm, freely movable, and painless on palpation. Initial differential diagnosis of lymphoma included lymphoma, metastatic neoplasia, lymphoid hyperplasia, and lymphadenitis. In hematology and cytology tests, this case was suspected to lymphoma. Immunohistochemical staining of neoplastic lymph node revealed that nearly 20.00 – 25.00% of neoplastic cells were strongly positive for anti CD3, whereas they were negative for both CD20 and CD79a. According to the microscopic and immunohistochemical findings, a diagnosis of T cell lymphoma was made. The present report is the first case of multicentric lymphoma with ocular metastasis in a dog in Iran.
Small Animal Internal Medicine
Ghasem Farjanikish; Omid Dezfoulian; Hossein Mohammadi
Volume 9, Issue 3 , September 2018, , Pages 289-292
Abstract
A four-year-old male Persian cat was referred with three weeks history of progressive lameness due to a rigid osseous mass with 3.50×2.50×2.00 cm in dimensions in his left arm. In the histopathological evaluation of bone biopsy, two distinct populations of cells including multinucleated giant ...
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A four-year-old male Persian cat was referred with three weeks history of progressive lameness due to a rigid osseous mass with 3.50×2.50×2.00 cm in dimensions in his left arm. In the histopathological evaluation of bone biopsy, two distinct populations of cells including multinucleated giant cells and oval cells which embedded in a fibro-osseous stroma and surrounded by lamellar bone trabeculae were observed. At necropsy, multiple metastatic nodules with different sizes unveiled in the liver and spleen. Microscopically, those tumor cells which already described in the bone lesion were also infiltrated to the liver and spleen. The neoplastic cells had no immunoreaction to CD68, desmin, alpha smooth muscle actin, S100, CD20, CD3 and pancytokeratin, but only a few cells had reaction to vimentin were similar to fibroblasts.
Pathology
Alireza Yousefi; Farshid Sarrafzadeh-Rezaei; Siamak Asri-Rezaei; Amir-Abbas Farshid; Mehdi Behfar
Volume 9, Issue 2 , June 2018, , Pages 105-111
Abstract
Chitosan bears numerous properties, such as biocompatibility, biodegradability and non-toxicity making it suitable for use in different biomedical fields. Zinc (Zn) is required for fibroblasts proliferation and collagen synthesis as essential elements of wound healing. Its nanoparticles are well known ...
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Chitosan bears numerous properties, such as biocompatibility, biodegradability and non-toxicity making it suitable for use in different biomedical fields. Zinc (Zn) is required for fibroblasts proliferation and collagen synthesis as essential elements of wound healing. Its nanoparticles are well known for their capability to enhance wound healing by cell adhesion and migration improvement through growth factors-mediated mechanisms. Poor blood supply and unique histological characteristics of tendon make its regeneration always slow. Also, adhesion formation between tendon and its surrounding tissues is another problem for neotendon to return to its normal structure and functional activities. In this study, a novel tubular scaffold of zinc oxide (ZnO) nanoparticles loaded chitosan has been fabricated for tendon repair. Experimental complete tenotomy of deep digital flexor tendon in a rabbit model was done and scaffolds were placed in the transected area after two ends suturing. After four and eight weeks, adhesion formation around the tendons and tissue reaction to the scaffolds were evaluated macroscopically. Inflammation, angiogenesis and collagen fibers arrangement were also analyzed in histopathological evaluations. After eight weeks, the scaffolds were absorbed completely, adhesions around the tendon were decreased and there was no sign of significant tissue reaction and/or infection in histopathological analyses. The reduced adhesion formation, improved gliding function and better histopathological characteristics suggest this scaffold application as a potential therapy in treatment of tendon acute injuries.
Small Animal Surgery
Vahid Fathipour; Zohreh Khaki; Seyyed Mehdi Nassiri
Volume 9, Issue 1 , March 2018, , Pages 19-26
Abstract
Recently, matrix metalloproteinases (MMP), in particular the gelatinases MMP-2 and MMP-9, generally considered as tumor markers for clinical applications. A longitudinal 2-years follow-up survey was performed on dogs with cutaneous tumor. Serum samples were obtained from 22 dogs with different cutaneous ...
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Recently, matrix metalloproteinases (MMP), in particular the gelatinases MMP-2 and MMP-9, generally considered as tumor markers for clinical applications. A longitudinal 2-years follow-up survey was performed on dogs with cutaneous tumor. Serum samples were obtained from 22 dogs with different cutaneous tumors and 22 health dogs at the time of surgery and one month, three months and one year after surgery. Gelatin zymography, hematological and biochemical assessment were performed for all serum samples. The serum alkaline phosphatase activity in dogs with malignant tumors was significantly higher than that in dogs with benign tumors and control cases. Latent forms of MMP-2 and MMP-9 were detected in all of the tumor cases. Gelatin zymography showed active form of MMP-9 in 12 cases (three benign and nine malignant tumors) and active form of MMP-2 in one fibrosarcoma case. Serum activity of active-MMP-9 and total MMP-9 was significantly higher in dogs with cutaneous tumors than those in controls. Tumor cases had higher serum activity of active-MMP-9 rather than controls. MMPs and alkaline phosphatase activities in serum were decreased significantly after surgery. Only one case with perianal gland adenoma showed recurrence of tumor four months after surgery in which active form of MMP-9 had identified one month before recurrence. According to the findings, it will be useful to measure ALP, MMP-2 and MMP-9 activities in the serum of dogs with cutaneous tumor for determination of tumor behavior before surgical treatment.
Large Animal Internal Medicine
Mohammad Farajli Abbasi; Bahador Shojaei; Omid Azari
Volume 8, Issue 4 , December 2017, , Pages 361-363
Abstract
Congenital agenesis of lumbar vertebrae was diagnosed in a day-old female lamb based on radiology and clinical examinations. There was no neurological deficit in hindlimb and forelimb associated with standing disability. Radiography of the abdominal region revealed absence of lumbar vertebrae. Necropsy ...
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Congenital agenesis of lumbar vertebrae was diagnosed in a day-old female lamb based on radiology and clinical examinations. There was no neurological deficit in hindlimb and forelimb associated with standing disability. Radiography of the abdominal region revealed absence of lumbar vertebrae. Necropsy confirmed clinical and radiographic results. No other anomaly or agenesis was seen macroscopically in the abdominal and thoracic regions as well as vertebral column. Partial absence of vertebral column has been reported in human and different animal species, as an independent occurrence or associated with other organs anomalies. The latter has been designated as caudal regression syndrome. Vertebral agenesis may arise from irregularity in the differentiation of somites to the sclerotome or sclerotome to the vertebral primordium. Most of the previously reported cases of agenesis were related to the lumbosacral region, lonely or along with other visceral absences. This case was the first report of congenital agenesis of lumbar vertebrae in a lamb.
Clinical Pathology
Belal Pashaie; Rahim Hobbenaghi; Hassan Malekinejad
Volume 8, Issue 3 , September 2017, , Pages 185-193
Abstract
Cynodon dactylon (Bermuda grass) is a perennial plant traditionally used as an herbal medicine in many countries. In the present study, anti-atherosclerotic property of ethanolic extract of C. dactylon was investigated in the experimentally induced hypercholesterolemia in rats. In this study, 36 male ...
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Cynodon dactylon (Bermuda grass) is a perennial plant traditionally used as an herbal medicine in many countries. In the present study, anti-atherosclerotic property of ethanolic extract of C. dactylon was investigated in the experimentally induced hypercholesterolemia in rats. In this study, 36 male Wistar rats were selected and allocated into six groups (n = 6). The control group received a normal diet, sham group received a high cholesterol diet (HCD; 1.50% cholesterol and 24.00% fat) and other groups received a HCD and ethanolic extract of C. dactylon at low (100 mg kg-1), moderate (200 mg kg-1) and maximum (400 mg kg-1) doses via gavages. The last group received atorvastatin (10 mg kg-1) through gavage with a HCD. The study period for all groups was six months. At the end of this period, parameters including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were assessed in the blood samples. Additionally, histopathological and immunohistochemical examinations on coronary and aorta arteries sections were performed. The results showed an increase in vessels wall thickness and proliferation of smooth muscle cells in the HCD group, while these pathological changes were not seen in C. dactylon-treated groups. Treatment of HCD animals with C. dactylon positively changed lipid profile by lowering of TC, TG and LDL-C. The results indicate that C. dactylon prevents from early atherosclerotic changes in the vessels wall.
Pathology
Farhad Safarpoor Dehkordi; Farhang Tirgir; Yousef Valizadeh
Volume 8, Issue 3 , September 2017, , Pages 215-221
Abstract
Application of smoke condensate derived from an indirect heating of jennet feces (Sargin) had been recommended by Iranian ancient scientists as a therapeutic agent. The present study was done to evaluate the healing effects of Guajol® ointment on burn wound in rat. The Guajol® ointment was prepared ...
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Application of smoke condensate derived from an indirect heating of jennet feces (Sargin) had been recommended by Iranian ancient scientists as a therapeutic agent. The present study was done to evaluate the healing effects of Guajol® ointment on burn wound in rat. The Guajol® ointment was prepared from the smoke condensate of Sargin samples. Wistar Rats (n = 50) were randomized into six groups including normal saline, silver sulfadiazine and 1.25%, 2.50%, 5.00% and 10.00% concentrations of Guajol® ointment. Under general anesthesia, dorsum of the rats were shaved and burn wounds were created using hot plate. Area of wounds and percent of healing were measured. Normal saline had the highest area of wound, followed by 1.25% Guajol® and silver-sulfadiazine groups. The group treated with 5.00% Guajol® showed the highest percent of healing. Percent of healing in NS, SSD and 5.00% Guajol® ointment groups on day 21 were 38.47%, 75.00% and 98.51%, respectively. Microscopic examination of wounds sections of rats treated with 5.00% Guajol® showed more collagen fibers and fibroblasts cells on day 7. Wounds of 5.00% Guajol® treated group was covered with healthy epithelial and epidermis tissues and hair follicles on day 21. This was the first report of using Sargin to heal the burn wound of rat. Further studies are recommended for investigation of the other effects of Guajol® ointment and its possible application in medicine.
Pathology
Reza Sayrafi; Navideh Mirzakhani; Reza Mobaseri
Volume 8, Issue 3 , September 2017, , Pages 231-236
Abstract
The aim of this study was to evaluate the protective effects of the turmeric in comparison to vitamin E on bursal damages induced by salinomycin in broiler chickens. In this study, forty one day-old broiler chicks were randomly divided into four treatment groups: 1- basal diet as control, 2- ...
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The aim of this study was to evaluate the protective effects of the turmeric in comparison to vitamin E on bursal damages induced by salinomycin in broiler chickens. In this study, forty one day-old broiler chicks were randomly divided into four treatment groups: 1- basal diet as control, 2- basal diet plus salinomycin, 3- basal diet plus salinomycin (SLM) and vitamin E (Vit. E) and 4- basal diet plus salinomycin and turmeric powder. The chicks were treated for two weeks. At the end of the experiment, the bursal tissues were removed and fixed in 10% formalin solution. Tissue sections were stained with hematoxylin and eosin stain for histopathological studies. Light microscopic observations showed that, SLM diminished cortex thickness of bursal tissue, enhanced its medulla zone and caused severe lymphocytic necrosis. In addition, SLM led to fibrosis of interstitium along with sever edema of medulla zone in the bursal tissue of the chicken. Administration of Vit. E and TP significantly inhibited the SLM-induced derangements and comparing the Vit. E and TP showed no significant differences. The results of this study indicated that the turmeric may protect bursa of Fabricius against toxicity induced by salinomycin in chicks.
Pathology
Moosa Javdani; Mohammad Hashemnia; Zahra Nikousefat; Mohammad Ghasemi
Volume 8, Issue 3 , September 2017, , Pages 265-268
Abstract
Osteoma is an uncommon bone tumor in avian species and other animals. A 2-year-old male canary (Serinus canaria) with a history of an oval mass in the left wing for several months was examined. Radiographs showed a radio-opaque mass. Upon the bird’s owner request, the canary was euthanatized and ...
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Osteoma is an uncommon bone tumor in avian species and other animals. A 2-year-old male canary (Serinus canaria) with a history of an oval mass in the left wing for several months was examined. Radiographs showed a radio-opaque mass. Upon the bird’s owner request, the canary was euthanatized and submitted for necropsy. The histopathologic examination revealed numerous trabeculae consisting of both woven and lamellar bone covered by one to several rows of normal osteoblasts. The trabeculae were closely packed, having only small intertrabecular spaces which contained proliferating osteoblasts, sinusoids and myeloid tissue. Based on clinical, radiographic, and histopathologic findings, the mass was diagnosed as extraskeletal osteoma. To the best of authors’ knowledge, extraskeletal osteoma has not been reported in in avian species so far, and this is the first report of osteoma tumor in the birds. However, benign tumors of bones are extremely rare in the birds, osteoma should be considered as a differential diagnosis in the birds with bone lesions.
Pathology
Ghasem Farjanikish; Azizollah Khodakaram-Tafti; Omid Dezfoulian
Volume 8, Issue 3 , September 2017, , Pages 269-273
Abstract
Astrocytoma as one of the most common central nervous system (CNS) tumors is rarely reported in veterinary literature. A 7-year-old Persian Lori-Bakhtiari ewe was presented to the clinic with a two months history of progressive blindness, nystagmus to the right, bilaterally decreased pupillary reflexes, ...
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Astrocytoma as one of the most common central nervous system (CNS) tumors is rarely reported in veterinary literature. A 7-year-old Persian Lori-Bakhtiari ewe was presented to the clinic with a two months history of progressive blindness, nystagmus to the right, bilaterally decreased pupillary reflexes, head pressing and paddling. At necropsy, a whitish well-circumscribed mass with dimensions of 3.50×2.50×1.50 cm was observed in the dorsal parietal lobe of the left cerebral hemisphere. Microscopically, the mass was well-circumscribed and highly cellular, consisted of round to elongated cells with scant and vacuolated cytoplasm with few, flaccid processes. The nuclei were round to oval with densely stippled chromatin and indistinct nucleoli. Immunohistochemical analyses showed positive staining for vimentin, S100 and glial fibrillary acidic protein. Definitive diagnosis of cerebral protoplasmic astrocytoma was made on the basis of the histopathological and immunohistochemical findings. This type of neoplasm should be included in the differential diagnosis of CNS lesions in the sheep.
Pathology
Firoozeh Niazvand; Layasadat Khorsandi; Mohammadreza Abbaspour; Mahmoud Orazizadeh; Negar Varaa; Mahtab Maghzi; Kheironesa Ahmadi
Volume 8, Issue 2 , June 2017, , Pages 155-161
Abstract
Curcumin has been found to be very efficacious against many different types of diseases. However, the major disadvantage associated with the use of curcumin is its low systemic bioavailability. In the present study the protective effects of curcumin-loaded poly lactic-co-glycolic acid nanoparticles (nanocurcumin) ...
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Curcumin has been found to be very efficacious against many different types of diseases. However, the major disadvantage associated with the use of curcumin is its low systemic bioavailability. In the present study the protective effects of curcumin-loaded poly lactic-co-glycolic acid nanoparticles (nanocurcumin) against mono-iodoacetate-induced osteoarthritis in rats was investigated. Mono-iodoacetate was injected into right knee joints to induce osteoarthritis. In experimental groups, 14 days after injection of mono-iodoacetate, curcumin (200 mg kg-1) and nanocurcumin (200 mg kg-1) were gavaged, respectively, for two weeks. Then the rats were sacrificed and the right knee joints were removed and fixated in 10% formalin for histological assessments. Cellularity and matrix staining were significantly increased in articular cartilage of curcumin-treated animals compared to mono-iodoacetate group (p < 0.01). These effects were significantly (p < 0.01) more in nanocurcumin-treated animals. These results suggested that administration of nanocurcumin prevented the structural changes of articular cartilage in mono-iodoacetate modelofosteoarthritis in rats.
Pathology
Ghasem Farjanikish; Ahmad Oryan
Volume 8, Issue 1 , March 2017, , Pages 81-84
Abstract
Leydig cell tumor as a sex-cord stromal tumor is a relatively uncommon ovarian tumorin bitch. A 10-year-old female German Shepherd dog was presented because of protrusion of a large tumor-like mass of 16 × 14 × 7 cm in dimensions from her vagina. After stabilization of the patient, the mass ...
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Leydig cell tumor as a sex-cord stromal tumor is a relatively uncommon ovarian tumorin bitch. A 10-year-old female German Shepherd dog was presented because of protrusion of a large tumor-like mass of 16 × 14 × 7 cm in dimensions from her vagina. After stabilization of the patient, the mass was removed surgically and concurrent ovariohysterectomy was also performed. Macroscopically, the healthy tissue of the right ovary was totally replaced by a homogeneous, brown and firm mass. The neoplasm was well-circumscribed and nodular and it was clearly demarcated from the healthy tissue. Histological examination revealed the presence of solid sheets and acinar structures composed of polyhedral to elongated cells. The neoplastic cells had large, eosinophilic, and vacuolated cytoplasms with round to oval nuclei and expressed vimentin on immunohistochemical examination. These gross, microscopic and immuno-histochemical features are characteristics of ovarian Leydig cell tumor.
Pathology
Alireza Talebi; Jafar Taifebagherlu; Arian Sharifi; Fatemeh Delkhosh-Kasmaie
Volume 7, Issue 4 , December 2016, , Pages 353-355
Abstract
Spondylitis is a reemerging epidemic spinal infection in male broiler chickens (5 to 7 weeks of age) as well as broiler breeder roosters (15 to 18 weeks of age). Among various causative agents, Enterococcus species and in particular E. cecorum, a gram-positive bacterium as a gastrointestinal flora of ...
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Spondylitis is a reemerging epidemic spinal infection in male broiler chickens (5 to 7 weeks of age) as well as broiler breeder roosters (15 to 18 weeks of age). Among various causative agents, Enterococcus species and in particular E. cecorum, a gram-positive bacterium as a gastrointestinal flora of birds, have mostly been isolated. On late September 2015, a number of 10 weeks old roosters with characteristic clinical signs of lameness and hock-sitting posture were autopsied. During thorough general routine post-mortem examinations, abnormalities like nodular masses correlated well with the hock-sitting posture and posterior paresis/paralysis were observed in joint spaces on the caudal thoracic vertebral column (T6-T7) immediately anterior to the kidneys in all affected birds. At histopathological examinations, osteomyelitis with limited pathological lesions including mononuclear inflammatory cells infiltration and edema in spinal cord were seen and the infection was diagnosed as an acute spondylosis.
Large Animal Internal Medicine
Behrokh Marzban Abbasabadi; Aliakbar Ahmadzadeh; Shahab Ramezanpour; Amir Reza Hajati Ziabari
Volume 7, Issue 2 , June 2016, , Pages 177-179
Abstract
Dipygus is a teratological fetus with a double pelvis, genitals, and extremities. Congenital duplications in cattle are rare. Caudal duplication is more common in sheep and pigs while cranial duplications seem to be predominant in cattle.Asymmetric or parasitic conjoined twins consisting of an incomplete ...
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Dipygus is a teratological fetus with a double pelvis, genitals, and extremities. Congenital duplications in cattle are rare. Caudal duplication is more common in sheep and pigs while cranial duplications seem to be predominant in cattle.Asymmetric or parasitic conjoined twins consisting of an incomplete twin (parasite) attached to the body of a fully-developed twin (autosite).This report deals with a male Holstein calf with two extra limbs, in the pelvic region which were directed ventrally between the two normal hind limbs. The extra limbs were completely developed in one side and in other side just a bony mass were observed. So classification has been made as asymmetrical attached twins. The genital system was not affected and just one extra kidney-like structure was found. To the authors’ best knowledge, this is the first report of asymmetrical monocephalus dipygus (tetrapus dibrachius) in a male Holstein calf in Iran.
Pathology
Masoumeh Moradi-Arzeloo; Amir Abbas Farshid; Esmaeal Tamaddonfard; Siamak Asri-Rezaei
Volume 7, Issue 1 , March 2016, , Pages 47-54
Abstract
In the present study, we investigated the effects of histidine and vitamin C (alone or in combination) treatments against isoproterenol (a β-adrenergic receptor agonist)-induced acute myocardial infarction in rats. We used propranolol (a β-adrenergic receptor blocker) to compare the results. ...
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In the present study, we investigated the effects of histidine and vitamin C (alone or in combination) treatments against isoproterenol (a β-adrenergic receptor agonist)-induced acute myocardial infarction in rats. We used propranolol (a β-adrenergic receptor blocker) to compare the results. Rats were given intraperitoneal injections of histidine (40 mg kg-1) and vitamin C (40 mg kg-1) alone and combined daily for 21 days. Propranolol (10 mg kg-1) was orally administered daily for 10 days (from day 11 to day 21). Myocardial infarction was induced by subcutaneous injections of 150 mg kg-1 of isoproterenol at an interval of 24 hr on days 20 and 21. Blood and tissue samples were taken for histopathological and biochemical evaluations following electrocardiography recording on day 21. Isoproterenol elevated ST segment, increased heart weight, heart rate, serum activities of aspartate transaminase, lactate dehydrogenase, creatine kinase-MB and heart tissue content of malondialdehyde, and decreased R wave amplitude and superoxide dismutase and catalase activities of heart tissue. Necrosis, edema and inflammatory cells infiltration were observed in myocardial tissue sections. Our results indicated that histidine and vitamin C alone, and especially in combination prevent isoproterenol-induced cardiotoxicity and have similar protective effects with propranolol. Cardioprotective effects of histidine and vitamin C may be associated with their ability to reduce free radical-induced toxic effects.
